Density calibration function

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Density calibration function

Wayne Su
Hello everyone,

I am doing a project to move a program under NIH Image to ImageJ. However
there is a problem on density calibration. I used same Rodbard function on
ImageJ and NIH Image, but got the different result. It seems the NIH image
gave better result. My calibration table is following:

17.08    .038
85.3    .607
96.9    .616
113.86    .657
119.66    .686
132.95    .733
151.06    .793
155.47    .821
169.65    .882
172.86    .896
186.42    .973
191.11    1.008
205.52    1.109
221.93    1.27
227.23    1.361
234.72    1.492
239.85    1.611
240.49    1.632
246.08    1.869
252.81    2.799

Any thought? Thanks.

Wayne
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Re: Density calibration function

Wayne Rasband
I get pretty good fits when I paste this data set into the
Analyze>Tools>Curve Fitting window, delete the first point and last two
points, and then use either a 2nd degree polynomial or Rodbard
function. There is a screenshot at
<http://rsb.info.nih.gov/ij/images/curve-fits.gif>.

-wayne

> I am doing a project to move a program under NIH Image to
> ImageJ. However there is a problem on density calibration. I
> used same Rodbard function on ImageJ and NIH Image, but got
> the different result. It seems the NIH image gave better
> result. My calibration table is following:
>
> 17.08    .038
> 85.3    .607
> 96.9    .616
> 113.86    .657
> 119.66    .686
> 132.95    .733
> 151.06    .793
> 155.47    .821
> 169.65    .882
> 172.86    .896
> 186.42    .973
> 191.11    1.008
> 205.52    1.109
> 221.93    1.27
> 227.23    1.361
> 234.72    1.492
> 239.85    1.611
> 240.49    1.632
> 246.08    1.869
> 252.81    2.799
>
> Any thought? Thanks.