Determining cell area

[Sarwar, Rizwan]

Hi

 

We are growing primary cultures of rat ventricular myocytes. After the
experiment, we want to determine the area of the myocytes, and I am
currently doing this by drawing around each cell with my mouse on
ImageJ, and measuring the area (Ctrl+M). Is there a more efficient way
to do this do this that will:

 

(1)      allow me to select each cell individually (because this is a
primary culture, there are other cell types, and I am excluding all
"spindly" shaped cells)

(2)      give me the area for each individual cell to calculate means,
etc.

 

Thanks

Rizwan

 

[hidden email]

[hidden email]

 

 

[Jieqi Wang]

Hi,
You can try the "Yawi 2D" plugin http://yawi3d.sourceforge.net/), which
provides a enhanced version of magic wand for ImageJ. Read "INSTALL.txt"
for instruction and "CHANGELOG.txt" for feature list.

Jieqi

Albert Einstein College of Medicine
http://jieqiwang.googlepages.com

"Some men see things as they are and say why. I dream things that never were and say why not."        -- George Bernard Shaw



Sarwar, Rizwan wrote:

> Hi
>
>  
>
> We are growing primary cultures of rat ventricular myocytes. After the
> experiment, we want to determine the area of the myocytes, and I am
> currently doing this by drawing around each cell with my mouse on
> ImageJ, and measuring the area (Ctrl+M). Is there a more efficient way
> to do this do this that will:
>
>  
>
> (1)      allow me to select each cell individually (because this is a
> primary culture, there are other cell types, and I am excluding all
> "spindly" shaped cells)
>
> (2)      give me the area for each individual cell to calculate means,
> etc.
>
>  
>
> Thanks
>
> Rizwan
>
>  
>
> [hidden email]
>
> [hidden email]
>
>  
>
>  
>
>