Diameters of droplets from images of overlapping droplets

[jmcginnity]

Hi,
Is there a macro or plugin in ImageJ that can determine the diameters of individual droplets in an image containing overlapping droplets?  An example is shown in the attached Word doc.

Overlapping_droplets.doc

This image is from Mickler, M.,  Boecker, B.,  Bart, H.-J.,  “Drop swarm analysis in dispersions with incident-light and transmitted-light illumination”, Flow Measurement and Instrumentation, Vol.30,  2013, p. 81–89 .

Thanks,

Justin McGinnity

[Pål Baggethun]

You can try the Hough Transform for circles. There is a plugin available (http://rsb.info.nih.gov/ij/plugins/hough-circles.html). I have not tested it, however, and I do not know if it will fit your purpose.

Pål Baggethun
Research Engineer
Elkem Technology


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of jmcginnity
Sent: 7. januar 2014 16:12
To: [hidden email]
Subject: Diameters of droplets from images of overlapping droplets

Hi,
Is there a macro or plugin in ImageJ that can determine the diameters of individual droplets in an image containing overlapping droplets?  An example is shown in the attached Word doc.

Overlapping_droplets.doc
<http://imagej.1557.x6.nabble.com/file/n5006048/Overlapping_droplets.doc>

This image is from Mickler, M.,  Boecker, B.,  Bart, H.-J.,  “Drop swarm analysis in dispersions with incident-light and transmitted-light illumination”, Flow Measurement and Instrumentation, Vol.30,  2013, p. 81–89 .

Thanks,

Justin McGinnity



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[jmcginnity]

Hi Pal,

Thanks for the suggestion.  I have tried this but without success as I seem to get far too many results.

I'm now using the information gained from the Ultimate Points (Process-Bimary-Ultimate Points) which gives the radius and centre of each bubble in the image, even overlapping bubbles.  I have just posted a request as to how to convert the Ultimate Point data (position (x,y) and radius) into a plot of circles.

Regards,

Justin