Does the Stitching plugin only create a stack in one channel?

[Michael Johnson]

Hello all,

I'm moving smaller images from Imaris to Fiji to stitch them together and move them back to Imaris for analysis. However, after stitching in Fiji, the resulting stack of images is in a single channel and so limits my ability to analyze the image in Imaris. Is there any way for the stitching plugin to retain the channels that exist for the initial images?

Thanks,
Michael

[Niko Ehrenfeuchter]

Dear Michael,

The stitching plug-in does not reduce our split the channels.

Could you describe your work flow a bit more in detail?

Cheers
Niko

On 13. November 2014 23:47:09 MEZ, Michael Johnson <[hidden email]> wrote:

>Hello all,
>
>I'm moving smaller images from Imaris to Fiji to stitch them together
>and
>move them back to Imaris for analysis. However, after stitching in
>Fiji, the
>resulting stack of images is in a single channel and so limits my
>ability to
>analyze the image in Imaris. Is there any way for the stitching plugin
>to
>retain the channels that exist for the initial images?
>
>Thanks,
>Michael
>
>
>
>--
>View this message in context:
>http://imagej.1557.x6.nabble.com/Does-the-Stitching-plugin-only-create-a-stack-in-one-channel-tp5010434.html
>Sent from the ImageJ mailing list archive at Nabble.com.
>
>--
>ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Romain Guiet]

Hi Michael,

on Fiji you can try to save your image using the bioformats exporter, ics/ids format.
we observe that "basic" .tif files from fiji could be interpreted by Imaris as 1 channel image.

I hope it could help,

Cheers,

Romain

---------------------------------------------------------------
Dr. Romain Guiet
Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 19, AI 0140
CH-1015 Lausanne

Phone: [+4121 69] 39629
http://biop.epfl.ch/
---------------------------------------------------------------

________________________________________
De : ImageJ Interest Group [[hidden email]] de la part de Niko Ehrenfeuchter [[hidden email]]
Envoyé : vendredi 14 novembre 2014 10:30
À : [hidden email]
Objet : Re: Does the Stitching plugin only create a stack in one channel?

Dear Michael,

The stitching plug-in does not reduce our split the channels.

Could you describe your work flow a bit more in detail?

Cheers
Niko

On 13. November 2014 23:47:09 MEZ, Michael Johnson <[hidden email]> wrote:

>Hello all,
>
>I'm moving smaller images from Imaris to Fiji to stitch them together
>and
>move them back to Imaris for analysis. However, after stitching in
>Fiji, the
>resulting stack of images is in a single channel and so limits my
>ability to
>analyze the image in Imaris. Is there any way for the stitching plugin
>to
>retain the channels that exist for the initial images?
>
>Thanks,
>Michael
>
>
>
>--
>View this message in context:
>http://imagej.1557.x6.nabble.com/Does-the-Stitching-plugin-only-create-a-stack-in-one-channel-tp5010434.html
>Sent from the ImageJ mailing list archive at Nabble.com.
>
>--
>ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
Diese Nachricht wurde auf einem Mobiltelefon erstellt.

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