Hi Guenther,
Process>FFT>Custom filter works on stacks.
Take our FFT, make a mask from it that is 255 where wou want to pass
the frequencies and 0 where you want to block it (essentially the
same as you would do for normal filtering, but you have to change the
values both inside *and* outside of your roi). Then smooth it
slightly (3 times 'smooth' or Gaussian Blur with a small radius to
aviod ringing artifacts).
In contrast to normal 'Inverse FFT', for 'Custom Filter' you have to
take care about creating a symmetric filter, it won't be made
symmetric with respect to the origin.
Michael
________________________________________________________________
On 2 Mar 2009, at 07:45, Guenter Giese wrote:
> Dear list members,
>
> I have stacks of images with darkened regions (shadow-like stripes)
> running
> through (two main directions with angles only some degrees to
> orthogonal
> directions). On a single image, I run FFT, edit the power spectrum
> (clearing
> of a polygonal ROI), and then do inverse FFT.
>
> Is it possible to do automated FFT/ROI clearing/inverse FFT on a
> whole stack
> using a polygonal (predefined?) ROI for editing the power spectrum?
> Perhaps
> as a macro?
>
> Best,
>
> Guenter
>
> ------------------------------------------
> Dr. Guenter Giese
> Light Microscopy Facility Manager
> Dept. of Biomedical Optics
> MPI fuer Medizinische Forschung Jahnstr. 29
> D-69120 Heidelberg, Germany
> Phone (+49) 6221-486-360 (Fax: -325)
> e-mail:
[hidden email]
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