Hi,
I am looking for FRAP Profiler Plugin to download; but can not find it. Can I request for the plugin with the USER GUIDE? Thank you. Best regards Nagendra Babu T PhD Student Department of Pharmacology University of Cambridge Cambridge, UK |
Dear Babu,
Sorry! I wrote this as a bit of a hacked adaptation of Tony Collins’ original plugin. Alas, there is no user guide, but it’s on my to-do list thus summer. The key is that it uses the ROI manager to store the reference region and FRAP ROIs, and then runs the plugin, giving you options for one- or two-parameter exponential decay curve fits. You can get everything here, where there is also a sample large TIFF stack data set from my lab for download: http://worms.zoology.wisc.edu/research/4d/4d.html#frap The plugin assumes you know a bit about ImageJ (how to save data to a text file, what the ROI Manager is, etc.). Cheers, Jeff On Aug 13, 2015, at 11:00 PM, IMAGEJ automatic digest system <[hidden email]<mailto:[hidden email]>> wrote: Date: Thu, 13 Aug 2015 14:34:32 -0700 From: Nagendra Babu <[hidden email]<mailto:[hidden email]>> Subject: FRAP Profiler Plugin Hi, I am looking for FRAP Profiler Plugin to download; but can not find it. Can I request for the plugin with the USER GUIDE? Thank you. Best regards Nagendra Babu T PhD Student Department of Pharmacology University of Cambridge Cambridge, UK ---------------------------------------- Jeff Hardin Professor and Chair, Department of Zoology Director, Biology Core Curriculum University of Wisconsin 1117 W. Johnson St. Madison, WI 53706 voice: (608) 262-9634 fax: (608) 262-7319 email: [hidden email]<mailto:[hidden email]> web page: http://worms.zoology.wisc.edu -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Dear Jeff,
Many thanks for the reply and the plugin. I use ImageJ already; hence, I believe I should be able to figure out using the plugin without much hassle. Thanks again! Best regards Nagendra Babu On 2015-08-14 15:08, Jeff Hardin [via ImageJ] wrote: > Dear Babu, > Sorry! I wrote this as a bit of a hacked adaptation of Tony Collins’ > original plugin. Alas, there is no user guide, but it’s on my to-do > list thus summer. The key is that it uses the ROI manager to store the > reference region and FRAP ROIs, and then runs the plugin, giving you > options for one- or two-parameter exponential decay curve fits. > > You can get everything here, where there is also a sample large TIFF > stack data set from my lab for download: > http://worms.zoology.wisc.edu/research/4d/4d.html#frap [1] > > The plugin assumes you know a bit about ImageJ (how to save data to a > text file, what the ROI Manager is, etc.). > > Cheers, > Jeff > > On Aug 13, 2015, at 11:00 PM, IMAGEJ automatic digest system <[hidden > email] [2]<mailto:[hidden email] [3]>> wrote: > > Date: Thu, 13 Aug 2015 14:34:32 -0700 > From: Nagendra Babu <[hidden email] [4]<mailto:[hidden email] [5]>> > Subject: FRAP Profiler Plugin > > Hi, > > I am looking for FRAP Profiler Plugin to download; but can not find > it. Can > I request for the plugin with the USER GUIDE? > > Thank you. > > Best regards > Nagendra Babu T > PhD Student > Department of Pharmacology > University of Cambridge > Cambridge, UK > > ---------------------------------------- > Jeff Hardin > Professor and Chair, Department of Zoology > Director, Biology Core Curriculum > University of Wisconsin > 1117 W. Johnson St. > Madison, WI 53706 > voice: (608) 262-9634 > fax: (608) 262-7319 > email: [hidden email] [6]<mailto:[hidden email] [7]> > web page: http://worms.zoology.wisc.edu [8] > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html [9] > > ------------------------- > > If you reply to this email, your message will be added to the > discussion below: > http://imagej.1557.x6.nabble.com/FRAP-Profiler-Plugin-tp5014021p5014032.html > [10] > To unsubscribe from FRAP Profiler Plugin, click here [11]. > NAML [12] > > Links: > ------ > [1] http://worms.zoology.wisc.edu/research/4d/4d.html#frap > [2] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=0 > [3] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=1 > [4] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=2 > [5] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=3 > [6] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=4 > [7] > http://webmail-3.hermes.cam.ac.uk/user/SendEmail.jtp?type=node&node=5014032&i=5 > [8] http://worms.zoology.wisc.edu > [9] http://imagej.nih.gov/ij/list.html > [10] > http://imagej.1557.x6.nabble.com/FRAP-Profiler-Plugin-tp5014021p5014032.html > [11] > [12] > http://imagej.1557.x6.nabble.com/template/NamlServlet.jtp?macro=macro_viewer&id=instant_html%21nabble%3Aemail.naml&base=nabble.naml.namespaces.BasicNamespace-nabble.view.web.template.NabbleNamespace-nabble.view.web.template.NodeNamespace&breadcrumbs=notify_subscribers%21nabble%3Aemail.naml-instant_emails%21nabble%3Aemail.naml-send_instant_email%21nabble%3Aemail.naml -- Nagendra Babu Thillaiappan PhD Student - Taylor's Lab Department of Pharmacology University of Cambridge Tennis Court Road Cambridge CB2 1PD United Kingdom |
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