FW: 3D Cluster Determination with Spatial Analysis

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FW: 3D Cluster Determination with Spatial Analysis

BirthingXS
The address didn't work for some reason. I'll try this...

-----Original Message-----
From: Xin Shen
Sent: Thursday, December 01, 2016 9:57 AM
To: '[hidden email]'
Subject: 3D Cluster Determination with Spatial Analysis

Hi Thomas,

This is Xin and I've been posting on the forums regarding cluster analysis. The basic idea again is as follows:

1. Take an image of a 3D stack of objects, run 3D Object Counter to determine the number of objects (and assign a number to each).

2. Take the same image, but place a Roi around each original cluster, expand the Roi by a set distance to incorporate some of the neighboring clusters.

3. Question is, is there a way to determine which of the original clusters has now been included within the newly expanded Roi? The output would be something like:

New Cluster 1
Object 1
Object 2
New Cluster 2
Object 3
Object 4

I've attached an example image for you to test, also a copy of the macro for enlarging the roi in 3D. Appreciate your time!

Cheers,
Xin

Points 1test3d-1.tif (4M) Download Attachment
Enlarge_3D_ROI.ijm (3K) Download Attachment