FW: Little help with VolumeJ

[Abramoff-Michael]

 I copied your message (without your address) to the archives so ImageJ users can see it. I hope you agree. Thanks, Michael Abramoff

-----Original Message-----
From: Leon Espinosa [mailto:[hidden email]]
Sent: Friday, October 28, 2005 10:21 AM
To: Abramoff-Michael
Subject: RE: Little help with VolumeJ

Thank you for your message, I have found the problem: I was using "gradient no index" instead "gradient +index (spectrum)"
so it works !!

Thank you

All the best, Leon


A 12:58 26/10/2005 -0500, vous avez écrit :

>Can you send me your RGB stack so I can try. I tried this many years
>ago and dont have  test set anymore? Thanks,
>
>Michael D. Abràmoff, MD, PhD
>Assistant Professor of Clinical Ophthalmology / Assistant Professor of
>Electrical and Computer Engineering Department of Ophthalmology and
>Visual Sciences University of Iowa Hospital and Clinics, PFP 11290C 200
>Hawkins Drive Iowa City, IA 52242 USA
>
>Tel: [phone]. Secretary Diane Stephenson: [phone].
>Skype michael-abramoff [hidden email]
>
>
>
>-----Original Message-----
>From: Leon Espinosa [mailto:[hidden email]]
>Sent: Wednesday, October 19, 2005 4:27 AM
>To: Abramoff-Michael
>Subject: Little help with VolumeJ
>
>Dear Michael,
>
>I'm trying to reconstruct a volume from a confocal stack. There is two
>markers. red: the cell shape (lipids) and green micro-particles (into
>the cell).
>
>VolumeJ works perfect if the red channel and green channel are splited.
>But if I use the RGB stack only the red channel is render. I have tried
>several ways without results:
>
>- check the FAQ
>- transform the RGB stak in 8 bits color image (LUT)
>- exchange the red and green channels but I still obtaining only the
>red reconstruction
>- I have split the rgb stack and render the red channel and the green
>channel and merge the volume images (color / RGB merge) but this is not
>good because all the green objects are visible...
>
>Is there a standard procedure to use RGB stacks ? or... how to merge
>correctly the resltuts obtained from the separated channels ?
>
>I really appreciate your help and grateful to you.
>
>Regards,
>
>Leon
>
>
>Leon Espinosa