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Hello
I am quite new to image j. But until now i have routinely been able to select to save confocal images as as TIFs (OIFs instead of the default confocal option of OIB). I have then gone to image j which has allowed me to select 'split channels' after which i have assigned colours, merged as i pleased and so on and so on. But I have confocal stacks which are 4 channels, and when i drag and drop the files containing the TIFs into image J, although it allows me to open the merged image; when i attempt to split it am informed that an images 'must be multichannel' in order to split.
Any advice? its taken months to get these images, and the samples are no longer usable to re image so i am a little panicked :)
cheers for your help guys!
ellen
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