|
|
Dear Image J users
There is what I am struggling with. I have one picture of cells stained with DAPI and second one stained for gH2AX. Using DAPI picture I am able to make mask of this picture and then create selection of each nucleus. This selection is than splited to individual ROIs for each cell nucleus. Having those splited ROIs I can use it to measure on second picture (gH2AX) separately for each splited ROIs, everything what is available in Set Measurements by using command Show all\Measure in ROI Manager. What I also want to calculate is the number of H2AX focci in each selection separately. One way to do this is using mask that was created on DAPI picture and use command Binary\ find maxima. But this command counts maxima on all ROIs together and does not show results when there are no distinguishable maxima (do not show 0 values). What I would like to do is counting those maxima in each ROI separately together with Mean Grey Value and other measurements from Set measurements.
Is there any way to do this? Like extend Set Measurements for Binary\find maxima?
Thanks in advance for any suggestions.
Pawel
|
Locked
