Fwd: [Hands-on] Super Resoultion Microscopy workshop at the NIH, October 15-17, 2014

[Justin Senseney-2]

---------- Forwarded message ----------
From: "Nardone, Mark (NIH/FAES) [T]" <[hidden email]>
Date: Sep 18, 2014 3:49 PM
Subject: [Hands-on] Super Resoultion Microscopy workshop at the NIH,
October 15-17, 2014
To: "[hidden email]" <[hidden email]>
Cc:

 SPONSORED BY

*FAES (Foundation for The Advanced Education in the Sciences, Inc.) at the
National Institutes of Health*

We are pleased to announce a new training workshop at the NIH, Bio-Trac 53
"Super Resolution in Microscopy"
<http://www.biotrac.com/pages/Tracs/Trac53.html> which will be offered on
October 15-17, 2014 at the NIH.

Dr. Xufeng Wu (NIH/NHLBI) has put together an excellent program, consisting
of lecture presentations given by active researchers from NHLBI, NIBIB, and
the HHMI; laboratory support from Dr. Hari Shroff's lab as well as other
core facilities on the campus. In this workshop, participants will
have *hands-on
access* to *six different platforms* throughout the three days of
instruction.



Each participant will receive a comprehensive binder containing all
material presented in the workshop (printed in color) along with laboratory
protocols and reference material.  There is a class limit of 30
participants with lab rotations containing a maximum of five participants
per station.



Registration information can be obtained from Ms. Carline Coote at FAES
(301-496-7975) or from the Bio-Trac website at www.biotrac.com, workshop
schedule and instructor information is listed below.



Further information on upcoming Bio-Trac workshops can be found on the calendar
link <http://www.biotrac.com/pages/calendar.html> on the Bio-Trac website.



*Please forward to others who might be interested *



All the best,

Mark

Mark Nardone
Biotechnology Training Contractor
Director Bio-Trac/CellServ Programs
at the NIH
301-496-8290

Bio-Trac, est. in 1985, has provided training to over 16,000 scientists and
is open to the scientific community.

Workshop Outline



*INTRODUCTION Super Resolution in Microscopy: Principles and Methods
October 15-17, 2014*

Wednesday, October 15, 2014

1. Introduction (Mark Nardone, FAES Bio-Trac) 9:00-9:15

2. Program Review (Xufeng Wu, Ph.D, NIH/NHLBI) 9:15-9:30

3. Perils and Pitfalls in Super-Resolution Microscopy (Hari Shroff, Ph.D,
NIH/NIBIB) 9:30 - 10:10

4. Study of focal adhesions using super resolution imaging (Clare
Water­man-Storer, Ph.D., NHLBI/NIH) 10:10- 10:50

5. Cell Biological Studies Using Super Resolution Microscopy (Jennifer
Lippincott-Schwartz, PH.D., NIH/NICHD) 11:00 - 11:45

Lunch 11:45 - 1:00

Laboratory 1:00 - 5:15

5. Introduction and Feature Highlights of the Instrument; Imaging
Acquisition Procedures; Sample Preparation Requirements and Recommendations
(Dye Choices and Imaging Solutions); Imaging Data Analysis and
Presentation; Trouble-shoot Common Problems Super in Resolution Imaging.

Laboratory Session I: 1:00-3:00

Laboratory Session II: 3:15-5:15

Thursday, October 16, 2014
Lecture 9:00 - 12:00

1. SIM Microscopy I - Theoretical Basis and Practical Guide: Structured
Illumination: Theory and Implementation; Image Reconstruction Algo­rithms;
Keys to a High-Quality Structured-Illumination Microscope (Lin Shao, Ph.D.,
HHMI/Janelia Farm) 9:00-9:40

2. SIM microscopy II - Theoretical Basis and Practical Guide: Digital
Mul­tifocal SIM (the Simplest Implementation); Analog Multifocal SIM (the
Fastest Implementation); Alternative Methods of Processing SIM Data (and
all Other Forms of Multi-Image Microscopy) (Andrew York, Ph.D., NIH/NIBIB)
9:45-10:25

3. PALM/dSTORM: Theoretical Basis and Practical Guide I; Overview of How
PALM/dSTORM Works; Differences Between PALM and dSTORM and Choosing Which
to Use; Typical Laser Intensities During Imag­ing ; What Probes are
Available; How to Pick a Good Probe; Optimizing dSTORM Reducing Buffer; How
to Interpret Your Final Image (Kem Sochacki, Ph.D., NIH/NHLBI) 10:30 - 11:10

4. PALM/dSTORM: Theoretical Basis and Practical Guide II; Is Your Data
Worth Fitting and Reconstructing? Methods for Fitting and
Reconstruction; How to Render the Final Image; Precision and Resolution
(Gerry Hammond, Ph.D., NIH/NICHD) 11:10 - 11:40

5. PALM/STORM Computer Lab: From Raw Data to Publication Quality Data With
ImageJ 11:15-12:45

Lunch 12:00 - 1:00

Laboratory 1:00 - 5:15

5. Introduction and Feature Highlights of the Instrument; Imaging
Acquisition Procedures; Sample Preparation Requirements and Recommendations
(Dye Choices and Imaging Solutions); Imaging Data Analysis and
Presentation; Trouble-shoot Common Problems in Super Resolution Imaging.

Laboratory Session V&VI: 1:00-3:00

Laboratory Session VII: 3:15-5:15

Friday, October 17, 2014
Lecture - 9:00 - 12:00 & 1:00 - 4:00

1. Advances in Fluorescent Protein Development and Application in Super
Resolution Microscopy (George Patterson, Ph.D., NIH/NIBIB) 8:30 - 9:10

2. STED Microscopy: Theoretical Basis and Practical Guide Basics and
Theory; Dyes Suitable for STED Imaging; Problems Associated with STED
Imaging; How STED Fits into the Toolbox of Super-Resolution Techniques
(Chris Combs, Ph.D., NIH/NHLBI) 9:10-9:50

3. Lattice Light Sheet Microscopy for 3D Live Cell Imaging (Wesley Legant,
Ph.D.) 10:00 to 10:30

4. Structured Illumination at TIRF Field for Live Cell Imaging (Dong Li,
Ph.D., NIH/NIA) 10:35- 11:05

5. PALM/STORM Computer Lab (Lunch Session): From Raw Data to Publication
Quality Data With ImageJ 11:15-12:45

Laboratory 1:00 - 5:15

5. Introduction and Feature Highlights of the Instrument; Imaging
Acquisition Procedures; Sample Preparation Requirements and Recommendations
(Dye Choices and Imaging Solutions); Imaging Data Analysis and
Presentation; Trouble-shoot Common Problems in Super Resolution Imaging.

Laboratory Session V & VI: 1:00-3:00

Laboratory Session VII: 3:15-5:15

Laboratory Stations
Station I
Leica – gSTED microscope, Model # TCS SP8
Geoff Daniels

Station II
GE – SIM microscope, Model # OMX V4
Katie O’Neil

Station III
Nikon – STORM microscope, Model # N-STORM
Eric Balzer

Station IV
Zeiss – PALM microscope, Model # ELYRA
Elise Shumsky

Station V
Hari Shroff LAB – Instant SIM Microscope
Andrew York

Station VI
Hari Shroff LAB – Dual-View Plane Illumination Microscope
Chenxi Wu

Station VII
Bob Fasulka – Leica GSD System, Model # GSD


 ------------------------------
 ------------------------------


*Staff List *Mark Nardone
Director
Bio-Trac Program
NIH

*Workshop Director*
Xufeng S Wu, Ph.D.
NIH/NHLBI

*Instructors*
Christian A Combs
NIH/NHLBI

Gerry Hammond, Ph.D.
NIH/NICHD

Wesley Legant, Ph.D.

Dong Li, Ph.D.
NIH/NIA

Jennifer Lippincott-Schwartz, Ph.D.
NIH/NICHD

George Patterson, Ph.D.
NIH/NIBIB

Lin Shao, Ph.D.
HHMI

Hari Shroff, Ph.D.
NIH/NIBIB

Kem Alyce Sochacki, Ph.D.
NIH/NHLBI

Justin W Taraska, Ph.D.
NIH/NHLBI

Clare Waterman-Storer, Ph.D.
NIH/NIHLB

Chenxi Wu
NIH/NIBIB

Andrew Gregory York, Ph.D.
NIH/NIBIB

*Laboratory Instructors *

Eric Balzer
Nikon Instruments

Bob Fasulka
Leica Microsystems

Katie O'Neil
GE Healthcare

Elise Shumsky
Carl Zeiss MicroImaging

Chenxi Wu
NIH/NIBIB Instant SIM Microscope

Andrew York
NIH/NIBIB
Dual-View Plane Illumination Microscope

Gerry Hammond, Ph.D
NIH/NICHD
Computer Lab

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