Dear Dorna,
Yes, you can form the JC1 ratio by using the image calculator to perform
the division and making sure to check the "32 bit" output option.
The "result" image will have ratio values.
Please be careful to look for movement of mitochondria between capture
of the green and red channels-- that will corrupt your ratio since
objects no longer line up correctly.
cheers,
On 5/22/2014 11:55 AM, Dorna wrote:
> ---------- Forwarded message ----------
> From: "Dorna" <
[hidden email]>
> Date: May 22, 2014 1:10 PM
> Subject: Request
> To: <
[hidden email]>
>
> Dear Image J Mailing List:
> I am a user of Image J software. i have a question about the use of this
> software to analysis of mitochondrial activity in vitrified mouse oocyte.
> * this is my question:*
> can we calculate *the ratio of orange and green **fluorescence(i*n JC1
> staining) using Image J software?
>
>
> *D.Sanaei*
> Ph.D. Candidate in cellular and developmental Biology
> Royan Institute
> Tehran-Iran, P.O.Box: 16635-148
> Tel: +98 21 23562720
> Fax:+98 21 22339923
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html--
__
Vytas Bindokas, Ph.D.
Research Assoc. (Assoc. Prof.),
Director, BSD Light Microscopy Core Facility
phone: 773-702-4875
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Dept Pharmacol Physiol Sci MC0926
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The University of Chicago
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shipping address (main KCBD site):
V. Bindokas
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