There seem to be a number of G ratio methods out there. Some people draw radii or myelin width by hand, others fit to a circle, some threshold and take the ratio of the square root of the axon area to the square root of axon plus myelin. For perfect circles all methods (example at
http://www.flickr.com/photos/39998519@N00/1428772522/ ) work fine. But cross sections of axons are not so neatly circular. And not all micrographs and segmentation methods are equal.
Are there datasets of axon cross sections which are considered gold standards that we could use to check other methods against?
For instance, within one study the relative differences may be conclusive, such as 0.45 vs 0.67. But do these numbers correspond accurately to the absolute results found in other studies? Maybe another method would have yielded 0.4 vs 0.6.
If there are standards out there, would very much like to use them. Please let us know!
Thank you!
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
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