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Dear All,
I have just started using imageJ and microscopy in general.
Therefore I have a very basic and stupid question for you XD
I am performing live single cell fluorescence microscopy with Nikon Biostation (cooled ccd camera).
my question is: Can I compare mean intensities between files that differ only in the gain with which I acquired the measurements?
I have the same setting for: exposure time, intensity of the lamp and resolution (600x800 binning). The gain is different: It´s 4.75 for one file and 5.65 for the other one.
I think that by subtracting the background then I should be able to compare between means..but I am not sure!
Thanks a lot
Elena
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