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Heat map from stack gives nul results

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Heat map from stack gives nul results

polykoz
1 post
Hi everyone!
I am trying to run Heat map from stack on my TIRF movies where I clearly see black background and white foci of target protein moving around or staying at the same spot. So theoretically speaking after using heatmap from stack plugin i should see areas of blue colors in places where my foci are stable and reddish zones where foci do not stay for long. However the only thing I get as an output of heatmap is uniform purple field without any dots.
Does anyone have any suggestions on what can be wrong with my analysis? Can it be formatting of the files?
Thank you!