Help analysis

[OBEID Patricia 154904]

Hello,
I work on understanding the mechanisms of cancerization. For this I use cell lines that have or not the ability to form functional 3D structures, the acini. Non-functional structures are called tumoroids.
During my tests I am compelled to calculate the percentage of acini. For this, I wrote a macro to have the total number of 3D structures but I have to manually count the acini because I have not found any way to discriminate them via software.
That's why I'm asking you today. Maybe you have some leads to tell me.
I enclose a photo of acinus, a photo of tumoroide and a typical image that I must analyze (brightfield).
In contrast to tumoroids, acini are partially or totally hollow structures (such as a ball).
I thank you for any help you can bring me.
Cordially
Patricia
_______________________________________
Patricia OBEÏD
Animateur Informatique Biomics
CEA Grenoble
DRF /IRIG/DS /BGE U1038/ Biomics / Bât 4020
17 rue des Martyrs
38054 GRENOBLE Cedex 9

Email : [hidden email]<https://webmail.cea.fr/owa/redir.aspx?SURL=zlCUMaAflr1ILBJzcvkjSniTvPLG8VQb5zwY2fTiPEoWL-VfZT_TCG0AYQBpAGwAdABvADoAcABhAHQAcgBpAGMAaQBhAC4AbwBiAGUAaQBkAEAAYwBlAGEALgBmAHIA&URL=mailto%3apatricia.obeid%40cea.fr>
Tél : [phone]          Fax : [phone]
http://www.cea.fr/drf/big/bge/biomics
https://twitter.com/BiomicsLab<https://webmail.cea.fr/owa/redir.aspx?SURL=KD06S-1P7GPcLz8UUmCQs-kGu50o5Qe7121aJj7K9q8WL-VfZT_TCGgAdAB0AHAAcwA6AC8ALwB0AHcAaQB0AHQAZQByAC4AYwBvAG0ALwBCAGkAbwBtAGkAYwBzAEwAYQBiAA..&URL=https%3a%2f%2ftwitter.com%2fBiomicsLab>
</

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html