|
|
I have a series of 30-slice scans (about 24) of abdomens of mice that I
would like to analyze for fat content.
I am trying to calculate the relative amount of visceral fat in each slice.
Right now, I am using the following technique: 1) draw an ROI around
non-Subcutaneous fat in the slice using the polygon tool 2) set a threshold
so that all non-fat is excluded (i.e., soft tissue, bone, etc.) 3) Analyze ->
Measure to get an estimate of the area.
This is working fine, but it is taking too long. Specifically, step 1 is taking a
while. When I draw the polygon after zooming in on the slice, I spend quite
some time adjusting the "dots" so that the border around the visceral compartment
is accurate and does not include any surface fat.
My question: is there a quicker way to do this with imageJ? Also, can I automate
this across slices, or do I have to manually draw an ROI, set the threshold, etc.
for 600 images?
Thanks!
Kapil Sachdeva
|
Locked
