Help needed - Analyzing non-binary particles

[Meghdad Saffaripour]

Hello,

We are doing research on the health effects of the soot produced by
different fuels. I need to measure the area and mean gray value of soot
particle images.
ImageJ can only analyze binary images. But if I convert my images to binary,
all the information about the thickness distribution of soot samples will be
deleted.
Do you know any methods for analyzing particles in non-binary
images?<http://n2.nabble.com/file/n2096839/soot13.tif>

I appreciate you help.

[Ben Tupper]

On Dec 31, 2008, at 2:58 PM, Meghdad Saffaripour wrote:

> Hello,
>
> We are doing research on the health effects of the soot produced by
> different fuels. I need to measure the area and mean gray value of  
> soot
> particle images.
> ImageJ can only analyze binary images. But if I convert my images  
> to binary,
> all the information about the thickness distribution of soot  
> samples will be
> deleted.
> Do you know any methods for analyzing particles in non-binary
> images?<http://n2.nabble.com/file/n2096839/soot13.tif>
>
> I appreciate you help.

Hi,

You can "redirect" measurements from your binary mask to the  
original.  See the "Redirect" option under the "Set Measuremnts"  
command.  See the docs here...

http://rsb.info.nih.gov/ij/docs/menus/analyze.html#set

Ben

[Wayne Rasband]

> Hello,
>
> We are doing research on the health effects of the soot produced by
> different fuels. I need to measure the area and mean gray value of  
> soot
> particle images.
> ImageJ can only analyze binary images. But if I convert my images to  
> binary,
> all the information about the thickness distribution of soot samples  
> will be
> deleted.
> Do you know any methods for analyzing particles in non-binary
> images?<http://n2.nabble.com/file/n2096839/soot13.tif>

The particle analyzer works with grayscale images that have been  
thresholded using the Image>Adjust>Theshold tool.

-wayne