Hi Jessica,
I next tried installing the LOCI Bioformats plugin. I managed to get the
stack to open as a hyperstack, but by using the colorized color mode and
split channels. However, I would like to see what the UCSD plugin used to
do - open the Fluoview stack as a merged, three-color image that I could
navigate by focal plane.
It should be possible to merge the colors by opening the data without the
"Split channels" option, then choosing Image > Type > RGB Color from the
menu once the data has been opened.
-Curtis
On Wed, Aug 11, 2010 at 9:59 PM, jesse_brann <
[hidden email]> wrote:
> Hello,
>
> I'm having a problem with ImageJ after the most recent Java update. I'm
> using the latest version of ImageJ (1.43) with the newest Java version
> (1.6.0_10).
>
> This problem happens on both our lab Macs (current operating system) and on
> a Windows computer with Windows XP Professional Version 2002 Service Pack
> 3.
>
> I can no longer open Fluoview images with the UCSD confocal microscopy
> plugin. I am attempting to open my confocal images (3-color z series, no
> time component). I've tried opening them with all of the various ImageJ
> File-Open menu options. Nothing works.
>
> I next tried installing the LOCI Bioformats plugin. I managed to get the
> stack to open as a hyperstack, but by using the colorized color mode and
> split channels. However, I would like to see what the UCSD plugin used to
> do - open the Fluoview stack as a merged, three-color image that I could
> navigate by focal plane. I am attempting to quantify co-localization, so
> the lack of the merged color is a problem.
>
> Thank you for any help you can provide - even simple instructions on how to
> properly open the Fluoview .tifs would be helpful!
>
> -Jessica
>
> Do you know how I can fix this?
>
> --
> View this message in context:
>
http://imagej.588099.n2.nabble.com/Help-with-Fluoview-imports-UCSD-plugin-LOCI-tools-tp5414776p5414776.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
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