Help with ImageJ Data Analysis

[Lacey Elwyn]

Dear Members of Image J Mailing List,
 
I am e-mailing with hopes in speaking with someone who is an expert in the ImageJ program. I am analyzing data and need some assistance. I am looking at catecholamine-containing nerves of the uterine artery using the glyoxylic acid technique, visualizing them under fluorescence microscopy. I am interested in using ImageJ to quantify the nerve fibers in a designated area of the artery so I can compare the innervation of different samples. If you could help me, I would greatly appreciate it. I look forward to hearing from you.
 
Sincerely,
 
Lacey Beth Elwyn, MS-II.
Department of Physiology
Chicago College of Osteopathic Medicine, Midwestern University.

[dscho]

Hi,

On Thu, 10 Jun 2010, Lacey Elwyn wrote:

> I am e-mailing with hopes in speaking with someone who is an expert in
> the ImageJ program. I am analyzing data and need some assistance. I am
> looking at catecholamine-containing nerves of the uterine artery using
> the glyoxylic acid technique, visualizing them under fluorescence
> microscopy.

Please realize that in not providing any concrete data, you made it
impossible to help you.

Hth,
Johannes

[dpoburko]

Hi Lacey,

   Can you post an example image (or link to one) and give a little more
detail about what kind of information you need to extract? When you say
"quantify the nerve fibres" this could mean a number of things. Do you
want the number fibres? Length of fibres? etc. I'm sure there are a
number of people here that will help you get on your way.

Cheers,
Damon

On 6/10/2010 11:18 AM, Lacey Elwyn wrote:

> Dear Members of Image J Mailing List,
>
> I am e-mailing with hopes in speaking with someone who is an expert in the ImageJ program. I am analyzing data and need some assistance. I am looking at catecholamine-containing nerves of the uterine artery using the glyoxylic acid technique, visualizing them under fluorescence microscopy. I am interested in using ImageJ to quantify the nerve fibers in a designated area of the artery so I can compare the innervation of different samples. If you could help me, I would greatly appreciate it. I look forward to hearing from you.
>
> Sincerely,
>
> Lacey Beth Elwyn, MS-II.
> Department of Physiology
> Chicago College of Osteopathic Medicine, Midwestern University.
>