Hi All,
I am new to java and doesn't really understand much about it. I am attaching herein java code for your reference that was generated after recording macro.
I particularly need help with two of these things:
1) Making ROI more flexible such that if there is a rectangular image then rectangular Roi covering the 95% of the image is selected. If there is circular image as in the case of agar plate, then Oval ROI covering 95% of the image can be selected.
2) Making redirect option more generic such that the grayscale image opened on imagej is selected in the redirect option.
Thanks a lot,
Rohitesh
import ij.*;
import ij.process.*;
import ij.gui.*;
import java.awt.*;
import ij.plugin.*;
public class My_Plugin implements PlugIn {
public void run(String arg) {
ImagePlus imp = IJ.getImage();
imp2 = imp.duplicate();
//IJ.setTool("oval");
imp.setRoi(new OvalRoi(203,34,172,377));
imp.setRoi(new OvalRoi(40,34,335,377));
imp.setRoi(new OvalRoi(40,34,374,377));
imp.setRoi(new OvalRoi(40,34,374,389));
IJ.setAutoThreshold(imp, "Default");
Prefs.blackBackground = false;
IJ.run(imp, "Convert to Mask", "");
IJ.run("Close");
IJ.run("Set Measurements...", "area mean integrated add redirect=colony.jpg decimal=3");
IJ.run(imp, "Analyze Particles...", " show=Outlines display exclude summarize");
}
}
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