Help with thresholding and analysis

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Help with thresholding and analysis

vormies1234
Hi community,

I'm relatively new to imageJ and am having a bit of trouble finding the right plugin and method for my analysis.

I have a stack of 2 channels. One channel (GFP) is used for thresholding/localising cell structures, and I want to re-use these same areas (GFP) to quantify my 2nd channel (Cy3).

Can anyone suggest a plugin and instructions that can do this for a stack?

Thanks in advance!