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Hello,
I have been trying to quantify the fluorescence of projections branching out of cell bodies.
I have imaged osteocytes (cells which are buried inside the skeleton) which have this long processes just like neurons (to make it simple!)
Now what I want to do is quantify the fluorescence from these processes excluding the fluorescence coming from the cell body. What I tried to do is to: 1)measure the whole area of my picture; 2)then draw ROI around each single cell body and measure them and finally 3)subtract the measurements of the cell bodies from the measurement of whole area so at the end I should only have measurement of fluorescence of my projections. Does it make any sense? Please help! or is there any automatic way I can select ROI and tell the program to exclude these area from the measurement?
Thanks
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