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Hello all,
I am acquiring my Zstack images with a Leica LSM710 using the program ZEN that saves the images in LSM format. Then I want to measure co-localization of 2 out of my 4 channes with IMARIS.
However, prior to my analysis in IMARIS, I would like to edit them in ImageJ to delete some areas (by using edit>clear selection, I can delete the nucleus out of my analysis plane for example). However, after I do this, I am not able to save this file in a format that I can open it in IMARIS. I tried several formats, and the ones that do open something, only one channel shows up instead of the 4.
Does anyone know how to do this?
Thank you very much
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