How to track changes in color composition along a Z-series?

[Lior Eshed]

Dear all,

I'm using IamgeJ to analyze images of a Biorad confocal microscope, to study the composition of bacterial Biofilms by differential fluoroscent staining.
I have a z-series set of 8-bit images in two colors (red and green). after merging them I get a series of RGB images with mostly yellow color (the result of merging red and green). However, this color varies between green to red, as a result of the dominant color in the specific Z cross-section.
I'm looking for a way to track these changes in the dominant color along the Z-series. There must be a simple way to do so... How?

Thanks!

Lior


************************************************************
" In wine there is wisdom, in beer there is strength.
   In water there is bacteria "

- German proverb
*************************************************************

[Gareth Edwards-3]

Hello,
I would suggest possibly using quantitative colocalisation, the 'Manders Coefficient' plugin that
comes with the WCIF version of Image-J is good-I'm using it to make a similar comparison at the
moment.
Best regards,
Gareth Edwards.


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http://www.garethedwards.fotopic.net

Dr Gareth O Edwards
Cardiff School of Biosciences
Cardiff University
Main Building
Park Place
Cardiff CF10 3TL
Wales UK

Email: [hidden email]
Phone: [phone] (main)
Phone: [phone] (office/lab)

>>> Lior Eshed <[hidden email]> 11/22/06 7:02 am >>>
Dear all,

I'm using IamgeJ to analyze images of a Biorad confocal microscope, to study the composition of
bacterial Biofilms by differential fluoroscent staining.
I have a z-series set of 8-bit images in two colors (red and green). after merging them I get a
series of RGB images with mostly yellow color (the result of merging red and green). However, this
color varies between green to red, as a result of the dominant color in the specific Z
cross-section.
I'm looking for a way to track these changes in the dominant color along the Z-series. There must
be a simple way to do so... How?

Thanks!

Lior


************************************************************
" In wine there is wisdom, in beer there is strength.
   In water there is bacteria "

- German proverb
*************************************************************