Identification of darkest X% of pixels

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Identification of darkest X% of pixels

balsop
Hello all,

Relatively new to ImageJ (but very impressed).  I'm trying to pick out the darkest PERCENTAGE of pixels in my grayscale images for some medical research on a new esophageal imaging technique, with the ultimate goal to change their color to highlight their location within the tissue.  I need the PERCENTAGE, because the absolute grayscale values differ from image to image.  Any ideas about how to do this?  I assume it has to do with a LUT but am unsure how to issue a percentage command and otherwise have been stuck with adjusting the threshold slider manually under each image...  Any suggestions would be much appreciated!!!

Ben