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Hi,
we need some help analysing our acquired images. We are testing a
cytotoxicity assay and compare different wells with fluorescent cells. The
cells are grown to a monolayer equally in all the wells and are thereafter
killed with different conditions. Thereby we end up with some wells still
harbouring a fluorescent monolayer and other wells containing only some
remaining fluorescent cells with big holes in between. To quantify this we
could start counting the remaining cells but this is not possible for the
wells still containing the monolayer so we cannot compare them. Therefore we
are looking for a way to get a black-white picture with the area covered by
a cell in white and the empty space in between in black. Then we would
estimate the cell-covered area and compare it in between the individual
wells. Could you please tell us the best way do achieve this?
Thanks a lot!
Sofia Domingues
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