Image processing

[degussa]

. I am really struggling  with  image addition and subtraction given me noisy / irreasonable results. I have three images (a) the reference image( dry  voids) (b) the time series image( partially filed void) (c) The saturated image( completely filled void). I perform the following  arithmetic

1. subtract image A from B to quantify the amount of  fluid in void after  a certain time
2. subtract image A from C to qualify the  voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of fluid in voids at time of acquiring image 2.

Problem
. When i do my subtraction, i  end up having negative pixels, whioch should not occur, how do i eliminate these pixels without compromising my  data

When i ratio  as described in step 3 above, i get values   above 1 which is not logical,  how do i remove values greater than 1 without compromising my data.  I attach 3 images  for anyone to please help me out. Thanks href="Reference-+dry+void.tif">Reference-+dry+void.tif8+hours+partially+filled+voids.tifSaturated-filled+voids.tif

[Han Gang Yu]

Almost done:
 
1) ST 5uM? turn off your Chinese software.
2) why a box on the black line for control?
3) replace 1000pA with 1 nA
HG

>>> On 5/28/2008 at 12:26 AM, in message <[hidden email]>, degus <[hidden email]> wrote:
. I am really struggling  with  image addition and subtraction given me noisy
/ irreasonable results. I have three images (a) the reference image( dry
voids) (b) the time series image( partially filed void) (c) The saturated
image( completely filled void). I perform the following  arithmetic

1. subtract image A from B to quantify the amount of  fluid in void after  a
certain time
2. subtract image A from C to qualify the  voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of
fluid in voids at time of acquiring image 2.

Problem
. When i do my subtraction, i  end up having negative pixels, whioch should
not occur, how do i eliminate these pixels without compromising my  data

When i ratio  as described in step 3 above, i get values   above 1 which is
not logical,  how do i remove values greater than 1 without compromising my
data.  I attach 3 images  for anyone to please help me out. Thanks
href="Reference-+dry+void.tif">Reference-+dry+void.tif
http://www.nabble.com/file/p17505032/8%2Bhours%2Bpartially%2Bfilled%2Bvoids.tif 
8+hours+partially+filled+voids.tif
http://www.nabble.com/file/p17505032/Saturated-filled%2Bvoids.tif 
Saturated-filled+voids.tif
--
View this message in context: http://www.nabble.com/Image-processing-tp17505032p17505032.html 
Sent from the ImageJ mailing list archive at Nabble.com.

[Han Gang Yu]

Please ignore the previous email which was not meant to send to the Group, but to my postdoc, instead. Sorry for the confusion.
 
HG

>>> On 5/28/2008 at 10:27 AM, in message <[hidden email]>, Han Gang Yu <[hidden email]> wrote:
Almost done:

1) ST 5uM? turn off your Chinese software.
2) why a box on the black line for control?
3) replace 1000pA with 1 nA
HG

>>> On 5/28/2008 at 12:26 AM, in message <[hidden email]>, degus <[hidden email]> wrote:
. I am really struggling  with  image addition and subtraction given me noisy
/ irreasonable results. I have three images (a) the reference image( dry
voids) (b) the time series image( partially filed void) (c) The saturated
image( completely filled void). I perform the following  arithmetic

1. subtract image A from B to quantify the amount of  fluid in void after  a
certain time
2. subtract image A from C to qualify the  voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of
fluid in voids at time of acquiring image 2.

Problem
. When i do my subtraction, i  end up having negative pixels, whioch should
not occur, how do i eliminate these pixels without compromising my  data

When i ratio  as described in step 3 above, i get values   above 1 which is
not logical,  how do i remove values greater than 1 without compromising my
data.  I attach 3 images  for anyone to please help me out. Thanks
href="Reference-+dry+void.tif">Reference-+dry+void.tif
http://www.nabble.com/file/p17505032/8%2Bhours%2Bpartially%2Bfilled%2Bvoids.tif 
8+hours+partially+filled+voids.tif
http://www.nabble.com/file/p17505032/Saturated-filled%2Bvoids.tif 
Saturated-filled+voids.tif
--
View this message in context: http://www.nabble.com/Image-processing-tp17505032p17505032.html 
Sent from the ImageJ mailing list archive at Nabble.com.

[Joachim Wesner]

Huh?

颠倒？范吨？


Mit freundlichen Grüßen / Best regards

小卫



                                                                           
             Han Gang Yu                                                  
             <[hidden email]>                                            
             Gesendet von:                                              An
             ImageJ Interest            [hidden email]                
             Group                                                   Kopie
             <[hidden email].                                            
             GOV>                                                    Thema
                                        Re: Image processing              
                                                                           
             28.05.2008 16:27                                              
                                                                           
                                                                           
              Bitte antworten                                              
                    an                                                    
              ImageJ Interest                                              
                   Group                                                  
             <[hidden email].                                            
                   GOV>                                                    
                                                                           
                                                                           




Almost done:

1) ST 5uM? turn off your Chinese software.
2) why a box on the black line for control?
3) replace 1000pA with 1 nA
HG

>>> On 5/28/2008 at 12:26 AM, in message <[hidden email]>,
degus <[hidden email]> wrote:
. I am really struggling  with  image addition and subtraction given me
noisy
/ irreasonable results. I have three images (a) the reference image( dry
voids) (b) the time series image( partially filed void) (c) The saturated
image( completely filled void). I perform the following  arithmetic

1. subtract image A from B to quantify the amount of  fluid in void after
a
certain time
2. subtract image A from C to qualify the  voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of
fluid in voids at time of acquiring image 2.

Problem
. When i do my subtraction, i  end up having negative pixels, whioch should
not occur, how do i eliminate these pixels without compromising my  data

When i ratio  as described in step 3 above, i get values   above 1 which is
not logical,  how do i remove values greater than 1 without compromising my
data.  I attach 3 images  for anyone to please help me out. Thanks
href="Reference-+dry+void.tif">Reference-+dry+void.tif
http://www.nabble.com/file/p17505032/8%2Bhours%2Bpartially%2Bfilled%2Bvoids.tif

8+hours+partially+filled+voids.tif
http://www.nabble.com/file/p17505032/Saturated-filled%2Bvoids.tif
Saturated-filled+voids.tif
--
View this message in context:
http://www.nabble.com/Image-processing-tp17505032p17505032.html
Sent from the ImageJ mailing list archive at Nabble.com.

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________