Image standardization

[Juliana]

I have taken adipose tissue images with 2 different microscopes, the 2 sets of images have different pixel ratio and area. The first set have images size in pixels are 2048 x 1536, the ratio is 2.9 pixels per um, the area in um is 706.21 x 529.66 um. The second set have images size in pixels of 1360 x 1024, the ratio is 0.9 pixels per um, the area in um is 1511.11 x 1137.78 um.

What I think is that the second set of images cover a bigger area in the tissue, but the images are lower resolution that those from the first set. But I am not completely sure of this

I managed to standardize the number of pixels in ImageJ, but I still have problems with the area, since both pixels and area are crucial in my research, I need to be able to compare between images. Any help in this issue will be much appreciated.

[jerie]

Hi Juliana,

You may wish to downsample your 2.9px per microm images if you want to
compare,  take a look at http://imagej.net/Downsample in this case.

Cheers,  jens

Visiting Scientist
Fundação Oswaldo Cruz, Centro de Desenvolvimento Tecnológico em Saúde
(CDTS), Rio de Janeiro,  Brazil.

Am 24.10.2016 03:47 schrieb "Juliana" <
[hidden email]>:

> I have taken adipose tissue images with 2 different microscopes, the 2 sets
> of images have different pixel ratio and area. The first set have images
> size in pixels are 2048 x 1536, the ratio is 2.9 pixels per um, the area in
> um is 706.21 x 529.66 um. The second set have images size in pixels of 1360
> x 1024, the ratio is 0.9 pixels per um, the area in um is 1511.11 x 1137.78
> um.
>
> What I think is that the second set of images cover a bigger area in the
> tissue, but the images are lower resolution that those from the first set.
> But I am not completely sure of this
>
> I managed to standardize the number of pixels in ImageJ, but I still have
> problems with the area, since both pixels and area are crucial in my
> research, I need to be able to compare between images. Any help in this
> issue will be much appreciated.
>
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.
> com/Image-standardization-tp5017453.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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