Hi Ahmed, may be you could use this ImageJ tool:
http://rsb.info.nih.gov/ij/docs/menus/analyze.html#gelsPlease, post some example images, in order to check if this could be useful in your case.
Best regards,
Rocco
Senior Microscopist
Crick Advanced Light Microscopy facility (CALM)
The Francis Crick Institute
1 Midland Road, NW1 1AT, London (UK)
https://roccodant.github.io/