Hi Dave,
Sorry for the delay in reply.
Image5D has bugs which I do not have time to fix. Do you need to use
Image5D specifically to accomplish your goals? If all you need is maximum
intensity projection, you could use the Image > Z Project... command on a
regular hyperstack.
The following macro works for me:
newImage("HyperStack", "8-bit color-mode label", 400, 300, 2, 59, 6);
run("Z Project...", "projection=[Max Intensity] all");
Regards,
Curtis
On Wed, Sep 30, 2015 at 4:42 PM, Knecht, David <
[hidden email]>
wrote:
> I was trying to project a dataset (2 channels, 59 z steps, 6 time points)
> in Image5D and if I do a Max Project, it does the first 5 time points
> correctly, but the 6th time point is blank. The data looks equivalent in
> each time point and all have the same number of slices and channels in the
> Image5D stack. Any suggestions as to how to make it do all time points?
> Thanks- Dave
>
> Dr. David Knecht
> Professor of Molecular and Cell Biology
> Core Microscopy Facility Director
> University of Connecticut
> 91 N. Eagleville Rd.
> Storrs, CT 06269
> 860-486-2200
>
>
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