ImageJ splitting 48bit TIFF images by default

[James Roberts (ABM ULHB - Medical Physics)]

Dear list,

As an extremely novice user of ImageJ I'm in need of some basic help!

My ImageJ has decided to open 48bit TIFF images into three separate RGB images in a stack by default. They are not true RGB images either as they look identical. See below,

[cid:image001.jpg@01CF1E7F.D4FDB3B0]

The last time I used ImageJ with 48bit TIFF images (few months ago) they opened by default in the original format and then I could manually split the channels via the Color menu which resulted in true R, G and B images each of 16bit.

Not sure what has changed?

Any advice would be much appreciated!


James






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[Rasband, Wayne (NIH/NIMH) [E]]

On Jan 31, 2014, at 7:27 AM, James Roberts (ABM ULHB - Medical Physics) wrote:

> Dear list,
>
> As an extremely novice user of ImageJ I'm in need of some basic help!
>
> My ImageJ has decided to open 48bit TIFF images into three separate RGB images in a stack by default. They are not true RGB images either as they look identical. See below,
>
> [cid:image001.jpg@01CF1E7F.D4FDB3B0]
>
> The last time I used ImageJ with 48bit TIFF images (few months ago) they opened by default in the original format and then I could manually split the channels via the Color menu which resulted in true R, G and B images each of 16bit.
>
> Not sure what has changed?

It is not possible to answer a question like this without seeing an example non-working image. What you should do is use a file sharing service like Dropbox to share one of your 48-bit TIFF images.

-wayne
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[dscho]

Hi Wayne,

On Fri, 31 Jan 2014, Rasband, Wayne (NIH/NIMH) [E] wrote:

> On Jan 31, 2014, at 7:27 AM, James Roberts (ABM ULHB - Medical Physics) wrote:
>
> > As an extremely novice user of ImageJ I'm in need of some basic help!
> >
> > My ImageJ has decided to open 48bit TIFF images into three separate RGB images in a stack by default. They are not true RGB images either as they look identical. See below,
> >
> > [cid:image001.jpg@01CF1E7F.D4FDB3B0]
> >
> > The last time I used ImageJ with 48bit TIFF images (few months ago) they opened by default in the original format and then I could manually split the channels via the Color menu which resulted in true R, G and B images each of 16bit.
> >
> > Not sure what has changed?
>
> It is not possible to answer a question like this without seeing an example non-working image. What you should do is use a file sharing service like Dropbox to share one of your 48-bit TIFF images.

Or use Fiji's Help>Upload Sample Image. Wayne, as always, I am more than
happy to be of assistance!

Ciao,
Johannes

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