ImageJ to measure blood vessel diameters

[Best, Derek]

Hello all,

This is my first time trying this, so be gentle...  

I'm new to the world of ImageJ, and programming in general, and I'm
trying to build or get a macro that can measure videotaped blood vessel
diameters that are 6 minutes long.  I take these 6 minute video tapes
and then digitize them.  I currently use a video caliper system but my
professor wants to move to ImageJ for diameter measurements.  Basically
I need a straight line to go across a region I define and measures that
length.  The diameter does change but there are long instances where the
diameter is the same.  How would I build this or has someone else built
this?

Derek Best
Dept. of Pharmacology and Physiology
University of Rochester

[Best, Derek]

 Basically
I need a straight line to go across a region I define and measures that
length. But I'm hoping for something that is a bit more automated such as
keeping the lumen defined or possibly the edge of one lumen defined?


 The diameter does change but there are long instances where the
diameter is the same.  How would I build this or has someone else built
this?

Derek Best
Dept. of Pharmacology and Physiology
University of Rochester

[Best, Derek]

 Basically
I need a straight line to go across a region I define and measures that
length.  But I have to measure this about 100 times in a movie type format.
Is there a macro that exists that I may possibly be able to modify to do
this?  

 The diameter does change but there are long instances where the
diameter is the same.  How would I build this or has someone else built
this?

Derek Best
Dept. of Pharmacology and Physiology
University of Rochester

[Bulent Celasun]

I have measured the mean diameter of a series of vascular lumina by
measuring the area of their lumen first. Then, assuming this as a perfect
circle, I have calculated the diameter for each. This method removes the
subjectivity of selecting an arbitrary line as the diameter. If your vessels
look physically "compressed", you can probably use the "perimeter" as a
starting point to calculate the diameter. (Assuming this perimeter as one of
a circle, and then...)
I can supply the simple macro I have prepared for this (from area to
radius).  However, I am sure there are better ways of doing your
measurement...

Bulent Celasun
Ankara, Turkey

[Best, Derek]

 But I need to do it on 1000 frames..  So I need an way to easily move the
line and reposition the line's length (at the push of button to either
extend or shorten) on consective frames.

Derek  

-----Original Message-----
From: Best, Derek
To: Best, Derek; ''''[hidden email]' ' ' '
Sent: 10/18/2005 2:49 PM
Subject: RE: ImageJ to measure blood vessel diameters

 Basically
I need a straight line to go across a region I define and measures that
length.  But I have to measure this about 100 times in a movie type
format.  Is there a macro that exists that I may possibly be able to
modify to do this?  

 The diameter does change but there are long instances where the
diameter is the same.  How would I build this or has someone else built
this?

Derek Best
Dept. of Pharmacology and Physiology
University of Rochester