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ImageJ / trackmate help

Martin Ward
3 posts
Hi,

My name is Martin Ward and I am a researcher from the university of  
Edinburgh.  I have been attempting to use image J and the Trackmate  
plugin to track particles with the aim of extracting useful values  
from their brownian motion (effective radius, diffusion etc.).  
However I have been unable to track the particles at all.  I have  
followed the tutorial, and all works well with the test data.  In  
appearence, my input looks very similar to the test data (bright white  
spots on a dark background), however when I get to the final stages no  
tracks are found.

I have spent the past couple of days playing around with the settings  
and levels of filtering etc, however this has not improved the  
situation.  I am not sure at all why this is happening, or indeed how  
to rectify the issue.  Any help at all on this will be greatfully  
received.  Also, if you require a sample of my input files, feel free  
to let me know and I can send something to show.

Also, I remain unclear on what the final output from Trackmate is?  
Will it, in the end, spit out the sort of values that I am looking for  
(Diffusivity, radius of gyration etc.) or must the plotted tracks then  
be further analysed to extract these values later?

Many thanks,
Martin Ward.

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