Hi Herbie,
I'm very sorry about that!
I was sure to have copied the link, but in fact forgot it...
So here is the link (and the plugin will be found at the end of the webpage):
https://www.lynceetec.com/types/downloads/Also I forwarded her request to the company so that one of their application engineer contacts Violeta in order to help her out for her needs.
Take care,
Philippe
----- Mail original -----
De: "l16" <
[hidden email]>
À: "imagej" <
[hidden email]>
Envoyé: Lundi 19 Avril 2021 13:29:46
Objet: Re: Images from Digital Holographic Microscopy
Bonjour Philippe,
all well here!
Thanks for the details!
"Lyncee Tec provides an ImageJ plugin under the following page [...]"
This may help the OP if the very page will follow...
Best
Herbie
:::::::::::::::::::::::::::::::::::::::::::::::::
Am 19.04.21 um 12:02 schrieb CARL Philippe (LBP):
> Servus Herbie,
> How are you doing? What's new?
> In another life I was selling the DHM (Digital Holographic Microscopy) microscope from the Lyncee Tec company.
> And from the phase measurements of a living cell with this microscope you can calculate its dry mass (this is based on a quite old Nature or Science paper) or derive the ion channel potential (i.e. equivalent to patch clamp measurements see
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051041).
> Violeta, how and where did you get your phase picture?
> Lyncee Tec provides an ImageJ plugin under the following page for reading their data.
> My best regards,
> Philippe
>
> Philippe CARL
> Laboratoire de Bioimagerie et Pathologies
> UMR 7021 CNRS - Université de Strasbourg
> Faculté de Pharmacie
> 74 route du Rhin
> 67401 ILLKIRCH
> Tel : +33(0)3 68 85 42 89
>
> ----- Mail original -----
> De: "l16" <
[hidden email]>
> À: "imagej" <
[hidden email]>
> Envoyé: Lundi 19 Avril 2021 11:18:51
> Objet: Re: Images from Digital Holographic Microscopy
>
> Greetings Violeta!
>
> "I would like to extract from .txt file statistical parameters [...]"
>
> Sorry, but it is far from clear what you want because you didn't attach
> the mentioned text file.
>
> The attached tif-image is a phase image (at least the gray-scale tells
> this) and you don't tell us *what* you would like to extract from it.
>
> I have no idea how "dry mass and morphological features of cells and
> nuclei" are related to this phase image.
>
> Please tell us more details about the task.
>
> Regards
>
> Herbie
>
> :::::::::::::::::::::::::::::::::::::::::::
> Am 18.04.21 um 19:04 schrieb Violeta Calin:
>> Dear all,
>>
>> I am looking for plugins in Image J to analyze quantitative phase images reconstructed from Digital Holographic Microscopy. This type of image has an attached .txt file, containing a phase shift value (in radians) for each pixel. I would like to extract from .txt file statistical parameters and specific parameters such as dry mass and morphological features of cells and nuclei.
>> Any help would be greatly appreciated.
>>
>> Violeta
>>
>>
>>
>> Violeta CALIN, PhD
>>
>> Dept. of Biophysics and Cell Biotechnology
>>
>> "Carol Davila" University of Medicine and Pharmacy
>> 8 Eroii Sanitari Blv., Sector 5
>> Bucharest, ROMANIA
>> www.umfcaroldavila.ro<
http://www.umfcaroldavila.ro/>
>>
>>
>> --
>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>>
>
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> ImageJ mailing list:
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Phase_snap.tif (977K)