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Immunohistochemistry

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Paul LeBourgeois

Immunohistochemistry

Has anyone used ImageJ for semi-quantitative IHC analysis? If so, how do
you go about it?

Thanks,

Paul LeBourgeois, MD < Medical Director

Asuragen, Inc. < 2150 Woodward, Ste. 100, Austin TX 78744

T: 512-585-2189 < F: 1-512-681-5201 < Web: www.asuragen.com
<http://www.asuragen.com/>

CONFIDENTIALITY NOTICE: The information in this e-mail and any
attachments is confidential and is intended for the addressee(s) only.
Reading, copying, disclosure or use by anybody else is unauthorized. If
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Ruy

Re: Immunohistochemistry

Try color deconvolution plugin:

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

Ruy Jaeger

========================================================================
   Dr. Ruy G. Jaeger, DDS, MSD, PhD
Professor
University of Sao Paulo Institute of Biomedical Sciences
   Department of Cell and Developmental Biology
   Av. Prof. Lineu Prestes 1524
Rooms 302 (Office and microscopy room) and 405 (Laboratory)
   Sao Paulo SP 05508-900
Phones: 55-11-30918454 (office), 30918034 (Lab) FAX 55-11-30917402
BRAZIL
website: http://www.icb.usp.br/~rgjaeger/index.html
========================================================================

On 10/7/08 6:00 PM, "Paul LeBourgeois" <[hidden email]> wrote:

> Has anyone used ImageJ for semi-quantitative IHC analysis? If so, how do
> you go about it?
>
>
>
> Thanks,
>
>
>
> Paul LeBourgeois, MD < Medical Director
>
> Asuragen, Inc. < 2150 Woodward, Ste. 100, Austin TX 78744
>
> T: 512-585-2189 < F: 1-512-681-5201 < Web: www.asuragen.com
> <http://www.asuragen.com/>
>
>
>
> CONFIDENTIALITY NOTICE: The information in this e-mail and any
> attachments is confidential and is intended for the addressee(s) only.
> Reading, copying, disclosure or use by anybody else is unauthorized. If
> you are not the intended recipient, please delete this message and any
> attachments and advise the sender by return e-mail.
>
>
>

Yan Gao

Re: Immunohistochemistry

In reply to this post by Paul LeBourgeois

All the time. Using colordeconvultion to quantify the brown intensity.

Yan Gao

Paul LeBourgeois <[hidden email]>
Sent by: ImageJ Interest Group <[hidden email]>
07/11/2008 02:01 PM
Please respond to
ImageJ Interest Group <[hidden email]>

To
[hidden email]
cc

Subject
Immunohistochemistry

Has anyone used ImageJ for semi-quantitative IHC analysis? If so, how do
you go about it?

Thanks,

Paul LeBourgeois, MD < Medical Director

Asuragen, Inc. < 2150 Woodward, Ste. 100, Austin TX 78744

T: 512-585-2189 < F: 1-512-681-5201 < Web: www.asuragen.com
<http://www.asuragen.com/>

CONFIDENTIALITY NOTICE: The information in this e-mail and any
attachments is confidential and is intended for the addressee(s) only.
Reading, copying, disclosure or use by anybody else is unauthorized. If
you are not the intended recipient, please delete this message and any
attachments and advise the sender by return e-mail.

_________________________

CONFIDENTIALITY NOTICE

The information contained in this e-mail message is intended only for the
exclusive use of the individual or entity named above and may contain
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under applicable law. If the reader of this message is not the intended
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prohibited. If you have received this communication in error, please
notify the sender immediately by e-mail and delete the material from any
computer. Thank you.

Shi, Zonggao

Re: Immunohistochemistry

Thank you very much, Dr Gao. I happened to be interested in this
application too. Could you please give me some details about
colordeconvolution? Is that a special plug-in? I didn't get it by
clicking around.

Thanks a lot,

Zonggao

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Yan Gao
Sent: Friday, July 11, 2008 3:15 PM
To: [hidden email]
Subject: Re: Immunohistochemistry

All the time. Using colordeconvultion to quantify the brown intensity.

Yan Gao

Paul LeBourgeois <[hidden email]> Sent by: ImageJ Interest
Group <[hidden email]>
07/11/2008 02:01 PM
Please respond to
ImageJ Interest Group <[hidden email]>

To
[hidden email]
cc

Subject
Immunohistochemistry

Has anyone used ImageJ for semi-quantitative IHC analysis? If so, how do
you go about it?

Thanks,

Paul LeBourgeois, MD < Medical Director

Asuragen, Inc. < 2150 Woodward, Ste. 100, Austin TX 78744

T: 512-585-2189 < F: 1-512-681-5201 < Web: www.asuragen.com
<http://www.asuragen.com/>

CONFIDENTIALITY NOTICE: The information in this e-mail and any
attachments is confidential and is intended for the addressee(s) only.
Reading, copying, disclosure or use by anybody else is unauthorized. If
you are not the intended recipient, please delete this message and any
attachments and advise the sender by return e-mail.

_________________________

CONFIDENTIALITY NOTICE

The information contained in this e-mail message is intended only for
the
exclusive use of the individual or entity named above and may contain
information that is privileged, confidential or exempt from disclosure
under applicable law. If the reader of this message is not the intended
recipient, or the employee or agent responsible for delivery of the
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly

prohibited. If you have received this communication in error, please
notify the sender immediately by e-mail and delete the material from any

computer. Thank you.

Gabriel Landini

Re: Immunohistochemistry

In reply to this post by Yan Gao

On Friday 11 July 2008, Yan Gao wrote:
> All the time. Using colordeconvultion to quantify the brown intensity.

Just curious, how do you calibrate your "quantification"?
E.g. "twice as darker" means... what?

G.

Yan Gao

Re: Immunohistochemistry

In reply to this post by Shi, Zonggao

Hi, Zonggao.

I am sorry reply your message till now. This is the web site you want to
down load your plugin.
http://www.uhnresearch.ca/facilities/wcif/fdownload.html
WCIF ImageJ bundle (~23Mb)

I generally create a mark up image to eliminate the unwanted cells or
tissues and then deconvoluted them.

Yan Gao

"Shi, Zonggao" <[hidden email]>
Sent by: ImageJ Interest Group <[hidden email]>
07/11/2008 05:07 PM
Please respond to
ImageJ Interest Group <[hidden email]>

To
[hidden email]
cc

Subject
Re: Immunohistochemistry

Thank you very much, Dr Gao. I happened to be interested in this
application too. Could you please give me some details about
colordeconvolution? Is that a special plug-in? I didn't get it by
clicking around.

Thanks a lot,

Zonggao

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Yan Gao
Sent: Friday, July 11, 2008 3:15 PM
To: [hidden email]
Subject: Re: Immunohistochemistry

All the time. Using colordeconvultion to quantify the brown intensity.

Yan Gao

Paul LeBourgeois <[hidden email]> Sent by: ImageJ Interest
Group <[hidden email]>
07/11/2008 02:01 PM
Please respond to
ImageJ Interest Group <[hidden email]>

To
[hidden email]
cc

Subject
Immunohistochemistry

Has anyone used ImageJ for semi-quantitative IHC analysis? If so, how do
you go about it?

Thanks,

Paul LeBourgeois, MD < Medical Director

Asuragen, Inc. < 2150 Woodward, Ste. 100, Austin TX 78744

T: 512-585-2189 < F: 1-512-681-5201 < Web: www.asuragen.com
<http://www.asuragen.com/>

CONFIDENTIALITY NOTICE: The information in this e-mail and any
attachments is confidential and is intended for the addressee(s) only.
Reading, copying, disclosure or use by anybody else is unauthorized. If
you are not the intended recipient, please delete this message and any
attachments and advise the sender by return e-mail.

_________________________

CONFIDENTIALITY NOTICE

The information contained in this e-mail message is intended only for
the
exclusive use of the individual or entity named above and may contain
information that is privileged, confidential or exempt from disclosure
under applicable law. If the reader of this message is not the intended
recipient, or the employee or agent responsible for delivery of the
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly

prohibited. If you have received this communication in error, please
notify the sender immediately by e-mail and delete the material from any

computer. Thank you.

_________________________

CONFIDENTIALITY NOTICE

The information contained in this e-mail message is intended only for the
exclusive use of the individual or entity named above and may contain
information that is privileged, confidential or exempt from disclosure
under applicable law. If the reader of this message is not the intended
recipient, or the employee or agent responsible for delivery of the
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please
notify the sender immediately by e-mail and delete the material from any
computer. Thank you.

drhbb

Re: Immunohistochemistry

I would be very appreciative if someone could tell me the steps for evaluating H DAB staining by using "color deconvolution" plugin of ImageJ.

I need to calculate the percentage of the brown cells.

I have already downloaded the program to my computer. 1. I opened the image and 2. separate the colors by using color deconvolution.However, I do not know the other steps.

Thank you,
Hbb