Importing Metafluor series

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Importing Metafluor series

Pedro J CamelloDr Pedro J Camello
Hi all,

I´m importing Metafluor (7.5.2) series (acquired with irregular intervals) to make time series measurements of discrete ROIs. It is easy to open all the images generated by Metafluor as a single stack using Import>Series Sequence (it gives directly a stack). However, in this way ImageJ doesn´t get the time acquisition for each frame, forcing me to open the associated Metafluor *.inf file to get the real timimng of each slice. Has anybody in the list made this task previously?

Thanks in advance
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Re: Importing Metafluor series

Melissa Linkert-2
Hi Pedro,

> I´m importing Metafluor (7.5.2) series (acquired with irregular intervals) to make time series measurements of discrete ROIs. It is easy to open all the images generated by Metafluor as a single stack using Import>Series Sequence (it gives directly a stack). However, in this way ImageJ doesn´t get the time acquisition for each frame, forcing me to open the associated Metafluor *.inf file to get the real timimng of each slice. Has anybody in the list made this task previously?

For what it's worth, there are plans to do this in Bio-Formats
(http://loci.wisc.edu/bio-formats):

http://trac.openmicroscopy.org/ome/ticket/4213

Unfortunately, it is not a terribly high priority, since we have too few
example datasets.  If you are willing and able to send a small dataset,
then we could almost certainly give this a much higher priority.

Regards,
-Melissa

On Sun, Feb 26, 2012 at 07:02:19AM -0500, Pedro J Camello wrote:
> Hi all,
>
> I´m importing Metafluor (7.5.2) series (acquired with irregular intervals) to make time series measurements of discrete ROIs. It is easy to open all the images generated by Metafluor as a single stack using Import>Series Sequence (it gives directly a stack). However, in this way ImageJ doesn´t get the time acquisition for each frame, forcing me to open the associated Metafluor *.inf file to get the real timimng of each slice. Has anybody in the list made this task previously?
>
> Thanks in advance