On 15 Jun 2006, at 19:36, Philip Ershler wrote:
> On Jun 15, 2006, at 7:57 AM, Van Hoomissen, Jacqueline wrote:
>
>> Greetings,
>>
>> I'm new to the list but I'm wondering if anyone out there is using
>> ImageJ for analysis of x-ray film from in situ hybridization. We
>> are currently doing in situ with rat brain and we want to analyze
>> our films with Image J. Can anyone suggest a good light box,
>> camera, and lens that works well with Image J for our purposes?
>>
>> Thanks
>>
>> Jacquie Van Hoomissen
>>
>> University of Portland
>
> Hi,
> I'd suggest using a flat bed scanner that is set up to scan
> transparencies. I think you'll get much better results than the
> light box camera approach.
>
> HTH,
>
> Phil
At least it is a cheaper way to get results at least as good. I've
done quite some in situs myself with 33P labelled probes. We have an
MCID set-up with dedicated lightbox and Dage CCD camera, but the
recently acquired Epson 4990 A4 flatbed scanner works just as well,
maybe even better. And it's a lot easier: instead of capturing images
of single sections, you can capture a whole film in one scan (approx
5 mins when scanning at 1600 ppi). Invest in a number of 14C
microscales from Amersham and you're up and running.
Bo
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