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I am trying to find a centroid on a frame, then integrate concentric circles until it hits the edge of the image or a selected region of interest. I will then either save a (radius, integrated pixel count) in a text file, or perhaps even do some fitting or analysis of the integrated pixel counts before exporting them.
I can do this by hand by selecting a circular ROI, stepping it out, and then keeping track of the total counts. However, this gets difficult for a single image, and I am looking to do it for multiple files with 50-1000 frames. It seems like there is probably a faster way to do it than with a macro, especially since I need to find the centroid for each frame, and output the results in a useful way. I think I will have to crack open my "Intro to Java" book to do it right.
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