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I'm doing immunohistochemistry and I have two different signals and want to do cell counting.
My idea was to use a z-stack (20 frames) in two dimensions (marker 1, marker 2). This is no problem for me to do for one image. However, the areas that I am looking at are big and I want to use a high magnification, so I want to combine different images into one very large image, which is also z-stacked in both markers, so the idea is to stitch the images which contain all the z-stack info and also both markers. I don't have a confocal microscope with a motorized stage which would make this easier to do, only an epifluorescence microscope.
Is this possible to do, any tips?
Thanks, Praz
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