Is it possible to save overlays in .tiff imagestacks?

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Is it possible to save overlays in .tiff imagestacks?

w00d
Hello community,

overlays in imagestack are not saved. After saving the imagestack via save as->image sequence->tiff (selected) I expected the overlays to be included into the save file.
But the overlays are missing.

The overlays were made via the "add to overlay" option in Analyze->Measure.

How can i save the overlays? Is this feature limited to a special type of .tiff?

The image below illustrates my issue:
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Re: Is it possible to save overlays in .tiff imagestacks?

Herbie-4
Noname,

it works here under Mac OSX with plain ImageJ (daily build 1.49o7).

Herbie

::::::::::::::::::::::::::::::
On 06.01.15 13:19, w00d wrote:

> Hello community,
>
> overlays in imagestack are not saved. After saving the imagestack via save
> as->image sequence->tiff (selected) I expected the overlays to be included
> into the save file.
> But the overlays are missing.
>
> The overlays were made via the "add to overlay" option in Analyze->Measure.
>
> How can i save the overlays? Is this feature limited to a special type of
> .tiff?
>
> The image below illustrates my issue:
> <http://imagej.1557.x6.nabble.com/file/n5011091/overlay_missing.png>
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Is-it-possible-to-save-overlays-in-tiff-imagestacks-tp5011091.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Is it possible to save overlays in .tiff imagestacks?

w00d
Hello Herbie,

I found out if the imagestack is saved as .tiff:
FILE -> SAVE AS -> .tiff the overlays are saved
if FILE -> SAVE AS -> Image Sequence -> .tiff overlays are missing.
(using 1.49m)

So i guess i have always to save the images in one .tiff.

Thanks
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Re: Is it possible to save overlays in .tiff imagestacks?

Rasband, Wayne (NIH/NIMH) [E]
> On Jan 6, 2015, at 7:44 AM, w00d <[hidden email]> wrote:
>
> Hello Herbie,
>
> I found out if the imagestack is saved as .tiff:
> FILE -> SAVE AS -> .tiff the overlays are saved
> if FILE -> SAVE AS -> Image Sequence -> .tiff overlays are missing.
> (using 1.49m)

The latest ImageJ daily build (1.49o13) fixes a bug that caused the File>Save As>Image Sequence command to not save overlays when saving in TIFF format.

-wayne

> --
> View this message in context: http://imagej.1557.x6.nabble.com/Is-it-possible-to-save-overlays-in-tiff-imagestacks-tp5011091p5011094.html
> Sent from the ImageJ mailing list archive at Nabble.com.

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