Is it possible to save your point selections onto images?

[Will Robbins]

Hi all,

does anyone know a way I can burn my point selections (used to measure
distances on the image) onto the .jpg that I'm looking at? The only
alternative as I see is to either use the pencil tool to mark where the
point selections were, or to do a screen capture and crop it down. As we
will be measuring literally thousands of images, these are both too
cumbersome techniques. I've done a bit of net searching, but am yet to
find a macro or plugin to help.

Many thanks,

Will

[Jacqueline Ross]

Hi Will,

Can't you just draw your selections onto the images using the ROI
Manager and then save the images as TIFFs? You can also save those
selections if you want to.

You may want to change the mode to RGB if the images are grayscale so
that you can draw the selections in a colour that stands out more.

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.health.auckland.ac.nz/biru/ 

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Will Robbins
Sent: 12 March 2007 15:57
To: [hidden email]
Subject: Is it possible to save your point selections onto images?

Hi all,

does anyone know a way I can burn my point selections (used to measure
distances on the image) onto the .jpg that I'm looking at? The only
alternative as I see is to either use the pencil tool to mark where the
point selections were, or to do a screen capture and crop it down. As we

will be measuring literally thousands of images, these are both too
cumbersome techniques. I've done a bit of net searching, but am yet to
find a macro or plugin to help.

Many thanks,

Will

[Will Robbins]

Hi Jacqui,

I have tried adding the points through the ROI manager, and while I can
measure the distances OK, I still have the same issue that it doesn't save
the points directly onto the image (even as a .TIF). Also the ROI file it
creates doesn't always place the points back onto the orginal image in the
exact same position when I re-load it (this is critical in what we're
measuring).

Cheers,

Will

[Wayne Rasband]

> does anyone know a way I can burn my point selections (used to
> measure distances on the image) onto the .jpg that I'm looking at?
> The only  alternative as I see is to either use the pencil tool to
> mark where the point selections were, or to do a screen capture
> and crop it down. As we will be measuring literally thousands of
> images, these are both too cumbersome techniques. I've done a
> bit of net searching, but am yet to find a macro or plugin to help.

Use the Edit>Draw command to permanently draw a point selection on  
the image. Double click on the point tool to set the mark width.

-wayne

[Will Robbins]

Fantastic! Thanks heaps for that!

Cheers,

Will




Rasband Wayne <[hidden email]>
Sent by: ImageJ Interest Group <[hidden email]>
12/03/2007 03:58 PM
Please respond to
ImageJ Interest Group <[hidden email]>


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[hidden email]
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Subject
Re: Is it possible to save your point selections onto images?






> does anyone know a way I can burn my point selections (used to
> measure distances on the image) onto the .jpg that I'm looking at?
> The only  alternative as I see is to either use the pencil tool to
> mark where the point selections were, or to do a screen capture
> and crop it down. As we will be measuring literally thousands of
> images, these are both too cumbersome techniques. I've done a
> bit of net searching, but am yet to find a macro or plugin to help.

Use the Edit>Draw command to permanently draw a point selection on
the image. Double click on the point tool to set the mark width.

-wayne



This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of their organisation.

[Jacqueline Ross]

Hi Will,

Sorry, I should have given more detailed instructions in my reply to you
but now Wayne has beaten me to it!

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.health.auckland.ac.nz/biru/ 

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Will Robbins
Sent: 12 March 2007 18:32
To: [hidden email]
Subject: Re: Is it possible to save your point selections onto images?

Fantastic! Thanks heaps for that!

Cheers,

Will




Rasband Wayne <[hidden email]>
Sent by: ImageJ Interest Group <[hidden email]>
12/03/2007 03:58 PM
Please respond to
ImageJ Interest Group <[hidden email]>


To
[hidden email]
cc

Subject
Re: Is it possible to save your point selections onto images?






> does anyone know a way I can burn my point selections (used to
> measure distances on the image) onto the .jpg that I'm looking at?
> The only  alternative as I see is to either use the pencil tool to
> mark where the point selections were, or to do a screen capture
> and crop it down. As we will be measuring literally thousands of
> images, these are both too cumbersome techniques. I've done a
> bit of net searching, but am yet to find a macro or plugin to help.

Use the Edit>Draw command to permanently draw a point selection on
the image. Double click on the point tool to set the mark width.

-wayne



This message is intended for the addressee named and may contain
confidential information. If you are not the intended recipient, please
delete it and notify the sender. Views expressed in this message are
those of the individual sender, and are not necessarily the views of
their organisation.

[Joachim Wesner]

Hi group,

is there a plugin/way to quickly simulate the impact of certain given MTFs
(defocus, spherical, ....) on certain given images?

Yes, I know I could write my macro or even Java-plugin to do that, but
maybe an almost ready-to-use solution already exists?


Mit freundliche Grüßen / Sincerely

Dr. Joachim Wesner
Leica Microsystems CMS GmbH
Abteilung FTO

Ernst-Leitz-Straße 17-37 | 35578 Wetzlar (Germany)
Tel. [phone] | Fax [phone]

Leica Microsystems CMS GmbH | GmbH mit Sitz in Wetzlar | Amtsgericht
Wetzlar  HRB 2432
Geschäftsführer: Dr. Martin Haase | Colin Davis | Dr. Wolf-Otto Reuter

http://www.leica-microsystems.com


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[Gluender]

Dear Joachim Wesner,

why not use "FFT > Custom Filter..." ?
(However, transfer functions are restricted to real-valued functions.)

>Hi group,
>
>is there a plugin/way to quickly simulate the impact of certain given MTFs
>(defocus, spherical, ....) on certain given images?
>
>Yes, I know I could write my macro or even Java-plugin to do that, but
>maybe an almost ready-to-use solution already exists?
>
>
>Mit freundliche Grüßen / Sincerely
>
>Dr. Joachim Wesner
>Leica Microsystems CMS GmbH
>Abteilung FTO

Best
--


                   Herbie

          ------------------------

          <http://www.gluender.de>

[Joachim Walter]

Hi Joachim,

Bob Dougherty's plugin Diffraction PSF 3D,
http://www.optinav.com/Diffraction-PSF-3D.htm, has an option to simulate
a spherical aberrated PSF, which you can convolve with your image stack
(Bob's plugin http://www.optinav.com/Convolve_3D.htm) to simulate the
effect of spherical aberration. See also his comments regarding
spherical aberration in the plugin description.

Best wishes,
Joachim



Joachim Wesner schrieb:

> Hi group,
>
> is there a plugin/way to quickly simulate the impact of certain given MTFs
> (defocus, spherical, ....) on certain given images?
>
> Yes, I know I could write my macro or even Java-plugin to do that, but
> maybe an almost ready-to-use solution already exists?
>
>
> Mit freundliche Grüßen / Sincerely
>
> Dr. Joachim Wesner
> Leica Microsystems CMS GmbH
> Abteilung FTO
>
> Ernst-Leitz-Straße 17-37 | 35578 Wetzlar (Germany)
> Tel. [phone] | Fax [phone]
>
> Leica Microsystems CMS GmbH | GmbH mit Sitz in Wetzlar | Amtsgericht
> Wetzlar  HRB 2432
> Geschäftsführer: Dr. Martin Haase | Colin Davis | Dr. Wolf-Otto Reuter
>
> http://www.leica-microsystems.com
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email 
> ______________________________________________________________________
>  

--
-----------------------------------------------
Dr. Joachim Walter

TILL I.D. GmbH
c\o BioImaging Zentrum
Großhaderner Str. 2
D-82152 Martinsried

Tel.: [phone]
Fax:  [phone]
 
Geschäftsführer: Dr. Rainer Uhl
HRB 129399 Amtsgericht München
USt.-IdNr. DE 205045721