Kymograph analysis

[dcjoshi]

Hi all
I am very new to image J and have a question about analysis of kymograph. I understood the concept of anterograde and retrograde direction of mobility. As mobile mitochondria makes diagonal lines on kymograph so how do we count total number of mobile mitochindria in a kymograph obtained using time series program ?

Dinesh C Joshi

[Arne Seitz]

Dear Dinesh,

a kymograph is a very versatile tool to measure the speed of moving structures.

To measure the directionality of the movement a kymograph is less well suited. It is possible but you will have to define rules how you to draw the line. In the case of a cell you can e.g. say to always draw the lines from the nucleus to the cell periphery. By doing so you can distinguish between retrograde and anterograde transport.

To my opinion a kymograph is not at all suited to measure the total number of moving structures. But maybe I have not completely understood what you are planning to do.

More details could be maybe helpful.

Cheers Arne

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> dcjoshi
> Sent: mardi 2 août 2011 17:27
> To: [hidden email]
> Subject: Kymograph analysis
>
> Hi all
> I am very new to image J and have a question about analysis of
> kymograph. I
> understood the concept of anterograde and retrograde direction of
> mobility.
> As mobile mitochondria makes diagonal lines on kymograph so how do we
> count
> total number of mobile mitochindria in a kymograph obtained using time
> series program ?
>
> Dinesh C Joshi
>
> --
> View this message in context:
> http://imagej.588099.n2.nabble.com/Kymograph-analysis-
> tp6645230p6645230.html
> Sent from the ImageJ mailing list archive at Nabble.com.

[Cammer, Michael]

If you treat a time series as a volume and use volume rendering, you often can see how the tracks interact and get a (rough) count of particles and paths.
-Michael

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Seitz Arne
Sent: Tuesday, August 02, 2011 3:12 PM
To: [hidden email]
Subject: Re: Kymograph analysis

Dear Dinesh,

a kymograph is a very versatile tool to measure the speed of moving structures.

To measure the directionality of the movement a kymograph is less well suited. It is possible but you will have to define rules how you to draw the line. In the case of a cell you can e.g. say to always draw the lines from the nucleus to the cell periphery. By doing so you can distinguish between retrograde and anterograde transport.

To my opinion a kymograph is not at all suited to measure the total number of moving structures. But maybe I have not completely understood what you are planning to do.

More details could be maybe helpful.

Cheers Arne



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[dcjoshi]

Hi Arne
Thanks a lot for the suggestion.
Actually I have a time series movie taken for the moving motochondria in sciatic nerve. It has many mitochondria and moving in antero as well as reto direction. I want to quantitate the number of mobile mitochondria from the kymograph.

Regards
Dinesh 

On 08/02/11, "Arne Seitz [via ImageJ]"   wrote:

>
>
>
>
> Dear Dinesh,
>
>
>
> a kymograph is a very versatile tool to measure the speed of moving structures.
>
>
>
> To measure the directionality of the movement a kymograph is less well suited. It is possible but you will have to define rules how you to draw the line. In the case of a cell you can e.g. say to always draw the lines from the nucleus to the cell periphery. By doing so you can distinguish between retrograde and anterograde transport.
>
>
>
> To my opinion a kymograph is not at all suited to measure the total number of moving structures. But maybe I have not completely understood what you are planning to do.
>
>
>
> More details could be maybe helpful.
>
>
>
> Cheers Arne
>
>
>
> > -----Original Message-----
>
>
> > From: ImageJ Interest Group [mailto:[hidden email](/user/SendEmail.jtp?type=node&node=6646186&i=0)] On Behalf Of
>
>
> > dcjoshi
>
>
> > Sent: mardi 2 août 2011 17:27
>
>
> > To: [hidden email](/user/SendEmail.jtp?type=node&node=6646186&i=1)
>
>
> > Subject: Kymograph analysis
>
>
> >
>
>
> > Hi all
>
>
> > I am very new to image J and have a question about analysis of
>
>
> > kymograph. I
>
>
> > understood the concept of anterograde and retrograde direction of
>
>
> > mobility.
>
>
> > As mobile mitochondria makes diagonal lines on kymograph so how do we
>
>
> > count
>
>
> > total number of mobile mitochindria in a kymograph obtained using time
>
>
> > series program ?
>
>
> >
>
>
> > Dinesh C Joshi
>
>
> >
>
>
> > --
>
>
> > View this message in context:
>
>
> > http://imagej.588099.n2.nabble.com/Kymograph-analysis-
> > tp6645230p6645230.html
>
>
> > Sent from the ImageJ mailing list archive at Nabble.com.
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
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