On 11/6/2012 7:04 AM, Maziar Shabestari wrote:
> Hi.
>
> Can anybody help me with live video acquisition from a Canon 5D Mark
> II? I would like to have live view of fluorescent bone sections which
> are obviously a very weak light source compared to what you have in
> conventional microscopy. The software package that came with the
> camera (EOS utilities) doesn't give me any customization options so in
> the live view mode I get only a black screen. I can take good pictures
> of the same section by having long exposure times though. Any
> suggestions what I can do?
>
> Thank you in advance.
>
> Regards,
>
This isn't really an ImageJ question, but your options are: 1) to using
binning and see if it helps; or 2) increase exposure time during live
capture; or 3) increase the gain on the camera.
Of course, the cost of the first is loss of pixel resolution; the cost
of the second is lowering the upper limit of capture rate in fps; and 3)
the cost of the third is increasing noise because it will be amplified
with your signal.
HTH,
Rob
--
__________________
Robert W. Baer, Ph.D.
Professor of Physiology
Kirksille College of Osteopathic Medicine
A. T. Still University of Health Sciences
Kirksville, MO 63501 USA
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