Make a picture combo of three color channel

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Make a picture combo of three color channel

jimmy28year@gmail.com
Hi everyone,

 

I got an color figure. I was trying to show my picture include the originial picutre with three different color.

 

My operation is done like this: 1.open image j and then open the figure; 2.click the image-color- split channels and got the three different channels picture. 3. Go to Stack-Image to Stack-Make montage.

Then i got a montage include three channel picture but without color showed. That is my question. What is the correct way to do?

 

 

Jimmy

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Re: Make a picture combo of three color channel

ctrueden
Hi Jimmy,

> Then i got a montage include three channel picture but without color
> showed. That is my question. What is the correct way to do?

1. Open your figure.

2. Run Image > Color > Make Composite. This converts your RGB color image
to an image stack with the desired lookup tables.

3. Move the new C slider onto the second (green) channel. This works around
a bug in ImageJ.

4. Run Image > Stacks > Make Montage.

You should now get the three channels side-by-side with the expected
colorization.

I don't think step #3 should actually be required, but if you omit it, the
first image of your montage will be the composite color depiction rather
than only the red channel for some reason.

Regards,
Curtis


On Tue, Apr 9, 2013 at 3:38 PM, [hidden email] <[hidden email]
> wrote:

> Hi everyone,
>
>
>
> I got an color figure. I was trying to show my picture include the
> originial picutre with three different color.
>
>
>
> My operation is done like this: 1.open image j and then open the figure;
> 2.click the image-color- split channels and got the three different
> channels picture. 3. Go to Stack-Image to Stack-Make montage.
>
> Then i got a montage include three channel picture but without color
> showed. That is my question. What is the correct way to do?
>
>
>
>
>
> Jimmy
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
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Re: Make a picture combo of three color channel

jimmy28year@gmail.com
Hi Curtis,

 

Thank you very much. It works like just you told me.

Now i have two picture, i want to make a combo including three channels of two picutures. That means i want to put six photo together. I make this because i want to compare two diffrent group cells. I know photoshop can do this, but i am wondering how to use image j to do this.

 

Could you help me?

 

 

Jimmy

 

 


Sent from Windows Mail


From: Curtis Rueden
Sent: ‎April‎ ‎9‎, ‎2013 ‎5‎:‎11‎ ‎PM
To: [hidden email]
Subject: Re: Make a picture combo of three color channel


Hi Jimmy,

> Then i got a montage include three channel picture but without color
> showed. That is my question. What is the correct way to do?

1. Open your figure.

2. Run Image > Color > Make Composite. This converts your RGB color image
to an image stack with the desired lookup tables.

3. Move the new C slider onto the second (green) channel. This works around
a bug in ImageJ.

4. Run Image > Stacks > Make Montage.

You should now get the three channels side-by-side with the expected
colorization.

I don't think step #3 should actually be required, but if you omit it, the
first image of your montage will be the composite color depiction rather
than only the red channel for some reason.

Regards,
Curtis


On Tue, Apr 9, 2013 at 3:38 PM, [hidden email] <[hidden email]
> wrote:

> Hi everyone,
>
>
>
> I got an color figure. I was trying to show my picture include the
> originial picutre with three different color.
>
>
>
> My operation is done like this: 1.open image j and then open the figure;
> 2.click the image-color- split channels and got the three different
> channels picture. 3. Go to Stack-Image to Stack-Make montage.
>
> Then i got a montage include three channel picture but without color
> showed. That is my question. What is the correct way to do?
>
>
>
>
>
> Jimmy
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
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Re: Make a picture combo of three color channel

ctrueden
Hi Jimmy,

> Now i have two picture, i want to make a combo including three
> channels of two picutures. That means i want to put six photo
> together.

You can do this using a macro. I wrote one for you:

    https://gist.github.com/ctrueden/5416605

If you have additional requirements, your best bet will be to read up on
the macro language. Use the Macro Recorder (Plugins > Macros > Record...)
to find out what the corresponding macro command is for each command in the
GUI. And use the Built-in Macro Functions page [1] for a rather
comprehensive list of things the macro language can do.

HTH,
Curtis

[1] http://imagej.net/developer/macro/functions.html


On Tue, Apr 9, 2013 at 5:31 PM, [hidden email] <[hidden email]
> wrote:

> Hi Curtis,
>
>
>
> Thank you very much. It works like just you told me.
>
> Now i have two picture, i want to make a combo including three channels of
> two picutures. That means i want to put six photo together. I make this
> because i want to compare two diffrent group cells. I know photoshop can do
> this, but i am wondering how to use image j to do this.
>
>
>
> Could you help me?
>
>
>
>
>
> Jimmy
>
>
>
>
>
>
> Sent from Windows Mail
>
>
> From: Curtis Rueden
> Sent: April 9, 2013 5:11 PM
> To: [hidden email]
> Subject: Re: Make a picture combo of three color channel
>
>
> Hi Jimmy,
>
> > Then i got a montage include three channel picture but without color
> > showed. That is my question. What is the correct way to do?
>
> 1. Open your figure.
>
> 2. Run Image > Color > Make Composite. This converts your RGB color image
> to an image stack with the desired lookup tables.
>
> 3. Move the new C slider onto the second (green) channel. This works around
> a bug in ImageJ.
>
> 4. Run Image > Stacks > Make Montage.
>
> You should now get the three channels side-by-side with the expected
> colorization.
>
> I don't think step #3 should actually be required, but if you omit it, the
> first image of your montage will be the composite color depiction rather
> than only the red channel for some reason.
>
> Regards,
> Curtis
>
>
> On Tue, Apr 9, 2013 at 3:38 PM, [hidden email] <
> [hidden email]
> > wrote:
>
> > Hi everyone,
> >
> >
> >
> > I got an color figure. I was trying to show my picture include the
> > originial picutre with three different color.
> >
> >
> >
> > My operation is done like this: 1.open image j and then open the figure;
> > 2.click the image-color- split channels and got the three different
> > channels picture. 3. Go to Stack-Image to Stack-Make montage.
> >
> > Then i got a montage include three channel picture but without color
> > showed. That is my question. What is the correct way to do?
> >
> >
> >
> >
> >
> > Jimmy
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html