Measure Colored Areas

[TinaS]

I have an image that shows the antigen staining. The antigen is stained by a
brown color, and I would like to know how to calculate the percentage or
total amount of brown spots (which are goblet cells) that are present in the
image. My first step, which is creating the image into a Binary image, skews
and blends the brown and blue (cell membrane) areas, so I'm not getting an
accurate reading on these values of Goblet cells. Neither the user guide nor
tutorial seems to shed light on this problem, does anyone have any
suggestions?

Thanks.

[Daniel James White]

Hi Tina,


On Jun 17, 2011, at 6:02 AM, IMAGEJ automatic digest system wrote:

>
> Date:    Thu, 16 Jun 2011 15:17:14 -0400
> From:    Tina Safavie <[hidden email]>
> Subject: Measure Colored Areas
>
> I have an image that shows the antigen staining. The antigen is stained by a
> brown color, and I would like to know how to calculate the percentage or
> total amount of brown spots (which are goblet cells) that are present in the
> image. My first step, which is creating the image into a Binary image, skews
> and blends the brown and blue (cell membrane) areas, so I'm not getting an
> accurate reading on these values of Goblet cells. Neither the user guide nor
> tutorial seems to shed light on this problem, does anyone have any
> suggestions?
>
> Thanks.

you need to seaprate the 2 stains by color deconvolution,
see

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

Gabriel did a nice job there of explaining it.

cheers

Dan

>

Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
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01307 DRESDEN
Germany

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