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Measure entire area inside a particle?

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Measure entire area inside a particle?

Broncosbeatall
7 posts
Hi everyone,

I'm trying to measure the area and diameter of rings of dye (from evaporated water). After thresholding, the image looks the one below. How can I calculate the total area inside the circle? It seems like using "analyze particles" will only give me the total area of thresholded spaces within the particle. Thanks in advance

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Re: Measure entire area inside a particle?

Michael Schmid
2136 posts
Hi anonymous,

one possibility with the current image; I'm not sure whether this would work for all of them (e.g. if the ring is ill-defined over a larger part, you will need some manual intervention like drawing its border there:

- split channels, delete all but the red one.
- Process>Filters>Maximum with a radius of 3.5 or 4 to close the circle
- Threshold 0-230
- Use the wand in 4-connected mode on some red pixel inside the circle
- Edit>Selection>Enlarge by approx. 4 pixels
- Measure to get the area


Michael
________________________________________________________________
On Jul 24, 2012, at 17:22, Broncosbeatall wrote:

> Hi everyone,
>
> I'm trying to measure the area and diameter of rings of dye (from evaporated
> water). After thresholding, the image looks the one below. How can I
> calculate the total area inside the circle? It seems like using "analyze
> particles" will only give me the total area of thresholded spaces within the
> particle. Thanks in advance
>
> http://imagej.1557.n6.nabble.com/file/n4999551/test.png 
>
>
>
> --
> View this message in context: http://imagej.1557.n6.nabble.com/Measure-entire-area-inside-a-particle-tp4999551.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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