Measurement of DNA with DAPI

[Manuel Bonilla]

I am trying to use Image J for measuring the amount of DNA in an algal cell. How can this be done?

Thanks for your help.

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[Gabriel Landini]

On Friday 21 August 2009, Manuel Bonilla wrote:
> I am trying to use Image J for measuring the amount of DNA in an algal
>  cell. How can this be done?

Have a look at this paper:

David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert, “From Pixels to
Picograms: A Beginners' Guide to Genome Quantification by Feulgen Image
Analysis Densitometry,” J. Histochem. Cytochem. 50, no. 6 (June 1, 2002):
735-750.

If I recall correctly the paper has a curious comment suggesting not doing
background correction. I am not sure that it is right, but the rest of the
paper is very informative.

G.

[dksamuel]

Thanks for a nice paper, Samuel, Bangalore, India

On Fri, Aug 21, 2009 at 12:57 PM, Gabriel Landini<[hidden email]> wrote:

> On Friday 21 August 2009, Manuel Bonilla wrote:
>> I am trying to use Image J for measuring the amount of DNA in an algal
>>  cell. How can this be done?
>
> Have a look at this paper:
>
> David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert, “From Pixels to
> Picograms: A Beginners' Guide to Genome Quantification by Feulgen Image
> Analysis Densitometry,” J. Histochem. Cytochem. 50, no. 6 (June 1, 2002):
> 735-750.
>
> If I recall correctly the paper has a curious comment suggesting not doing
> background correction. I am not sure that it is right, but the rest of the
> paper is very informative.
>
> G.
>