Measurements on multiple Concentric Rings associated with ROIs

[Declan James]

Hello List

I'm stuck.  I am using the FindMaxima plugin to identify many bright spots
in a time series stack. I then save all the maxima to ROI manager.  I
would like to place concentric rings (2 or 3) around each maxima.  I have
found the concentric ring plugin but it does not appear to be able to
position concentric rings around multiple ROI's.

Is there a way to position concentric rings around multiple ROIs?  

In my case I have a bright flash which radiates outward a bit like ripples
after throwing a stone (the maxima) into a pond.  I would like to measure
the average intensity for each ring around an ROI.

Can measurements be obtained from concentric circles associated with an
ROI?  

I would very much appreciate any help.  

Declan

[Michael Schmid]

Hi Declan,

the concentric circles plugin
   http://rsb.info.nih.gov/ij/plugins/concentric-circles.html
produces an overlay, thus pure graphics, not a roi that can be
used for measuring.

You would probably have to write a macro:
For each slice
- run Find Maxima with output Single Point
- run particle analyzer on the newly created image to get the
   coordinates
- close the new image
- get the coordinates from the Results Table and create the
   appropriate circular rois. Use setKeyDown("alt") and then make
   a smaller oval roi inside the outer one to deselect the inner
   disk. This gives you a ring.
   Use setKeyDown("shift") if you want to add another circle to
   the selection.
- do the measurement

Michael
________________________________________________________________

On 22 May 2007, at 02:27, Declan James wrote:

> Hello List
>
> I'm stuck.  I am using the FindMaxima plugin to identify many  
> bright spots
> in a time series stack. I then save all the maxima to ROI manager.  I
> would like to place concentric rings (2 or 3) around each maxima.  
> I have
> found the concentric ring plugin but it does not appear to be able to
> position concentric rings around multiple ROI's.
>
> Is there a way to position concentric rings around multiple ROIs?
>
> In my case I have a bright flash which radiates outward a bit like  
> ripples
> after throwing a stone (the maxima) into a pond.  I would like to  
> measure
> the average intensity for each ring around an ROI.
>
> Can measurements be obtained from concentric circles associated  
> with an
> ROI?
>
> I would very much appreciate any help.
>
> Declan