Measuring adipocyte cell size in suspension

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Measuring adipocyte cell size in suspension

Motku
I want to use automated programs such as imageJ to quickly measure adipocyte size. The adipose tissue has been digested with collagenase and the adipocytes are floating in saline for the microscopy pictures. Look at the attachment for an example picture. Is there an existing macro/program to quickly analyse the diameter and/or are of these cells? Most programs for imageJ (say http://sw.wikkii.com/wiki/Adiposoft) are used for stained histological sections. The adiposoft macro does not work for pictures in suspension that I want to analyse.

How should I proceed or is there a ready made macro for this?